IDENTIFICATION OF DIAGNOSTIC MOLECULAR MARKERS FOR GENETIC PURITY TESTING IN COWPEA [Vigna unguiculata (L.) Walp.]
| dc.contributor.advisor | NETHRA, N. | |
| dc.contributor.author | CHANDANA, B. S. | |
| dc.date.accessioned | 2018-09-11T09:34:02Z | |
| dc.date.available | 2018-09-11T09:34:02Z | |
| dc.date.issued | 2018-09-10 | |
| dc.description.abstract | Genetic purity is one of the important quality criteria required for successful seed production and seed certification. Hence, the experiment was set to evaluate the usefulness of SSR markers to determine genetic purity of commercial seed lots of cowpea, which are present in the seed chain of Karnataka. Out of 55 SSR markers studied, 10 SSR markers showed polymorphism for each of the 7 varieties, which revealed 25 alleles at 10 loci with an average of 2.5 alleles per locus. The SSR 6292 showed the highest number of alleles with the PIC value of 0.71. PKB-4 cultivar was found to be most divergent from dendrogram constructed by UPGMA clustering. The genetic purity of the seed lots was tested by following the 20 × 20 matrix, where the purity levels recorded cent per cent for all the seed lots except for KBC-2, IT-38956-1 and C-152 varieties, for which the purity levels are 98%, 96% and 97%, respectively. This analysis revealed that SSR marker is a potential tool for fingerprinting of varieties as well as for the genetic purity testing of the seed lots. Thus, it is helpful for delivering pure seed lots to the farmers. | en_US |
| dc.identifier.other | Th-12172 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810072686 | |
| dc.language.iso | en | en_US |
| dc.pages | 90 | en_US |
| dc.publisher | UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU | en_US |
| dc.sub | Seed Science and Technology | en_US |
| dc.subject | null | en_US |
| dc.theme | IDENTIFICATION OF DIAGNOSTIC MOLECULAR MARKERS | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | IDENTIFICATION OF DIAGNOSTIC MOLECULAR MARKERS FOR GENETIC PURITY TESTING IN COWPEA [Vigna unguiculata (L.) Walp.] | en_US |
| dc.type | Thesis | en_US |