COMPARISON OF VARIOUS METHODS OF ISOLATION AND IDENTIFICATION OF YERSINIA SPECIES IN MILK AND MILK PRODUCTS

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dc.contributor.authorPugazhenthi, TR
dc.contributor.authorNaresh Kumar, C
dc.contributor.authorElango, A
dc.contributor.authorDhanalakshmi, B
dc.contributor.authorRamaswamy, V
dc.contributor.authorAyyadurai, K
dc.contributor.authorTANUVAS
dc.date.accessioned2019-12-24T10:23:25Z
dc.date.available2019-12-24T10:23:25Z
dc.date.issued2010
dc.descriptionTNV_EJDS_2010_38(2)1en_US
dc.description.abstractOn biochemical characterization of suspected Yersinia species isolated from milk and milk products, twenty (l9.42%) were identified as Yersinia sp. by the conventional biochemical methods which include 9 Yersinia enterocolitica, 6 Y. frederiksenii and S Y. kfisrensenii where as, 18 by the commercial kit method which include 7 Y. emerocolitica, 6 Y frederiksenii and 5 Y kriszensenii Among the 29 presumptive Yersinia isolates tested in PCR assay, only nine were identified as Y entrrocolirim, as they showed amplification for species specific 16S rRNA gene (330 bp). In multiplex PCR, the same 4 pathogenic isolates harboured one to four virulent genes either single or in combination. The number of l’. enteroco/itica isolates identified in all these three methods was almost same, Molecular method identified more number of isolates than that of commercial kit method which may be due to the accuracy of PCR method. The results found clearly show that the molecular and conventional biochemical methods are equally reliable for detection of contamination of milk with Yersinia sp.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810138775
dc.keywordsMilk and milk products -Yersinia sp.- Biochemical and molecular methods — comparision.en_US
dc.language.isoenen_US
dc.pages1en_US
dc.relation.ispartofseries;2
dc.subjectVeterinary Scienceen_US
dc.titleCOMPARISON OF VARIOUS METHODS OF ISOLATION AND IDENTIFICATION OF YERSINIA SPECIES IN MILK AND MILK PRODUCTSen_US
dc.title.alternativeEgyptian Journal of Dairy Sciencesen_US
dc.typeArticleen_US
dc.volume38en_US
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