A COMPARATIVE STUDY ON CYTOGENETIC PROFILE OF CROSSBRED AND INDIGENOUS PIGS
| dc.contributor.advisor | SAKUNTHALA DEVI, K(MAJOR) | |
| dc.contributor.advisor | PUNYA KUMARI, B | |
| dc.contributor.advisor | SURESH, J | |
| dc.contributor.author | HARSHINI, V | |
| dc.date.accessioned | 2018-12-01T11:03:53Z | |
| dc.date.available | 2018-12-01T11:03:53Z | |
| dc.date.issued | 2017-02 | |
| dc.description.abstract | ABSTRACT: The present analysis was carried out on 30 blood samples of LWY crossbred pigs (15 females and 15 males) maintained at All India Coordinated Research Project on pig (AICRP), College of Veterinary Science, Tirupati, Chittor District of Andhra Pradesh and on 30 blood samples of indigenous pigs (15 females and 15 males) maintained by the farmers in and around Tirupati to study the cytogenetic profile of both the breeds and their morphometric measurements using short term leukocyte culture technique. The diploid chromosome number (2n) in both the breeds was 38 with XX and XY complement in females and males respectively and a fundamental number of 64 as in exotic pigs. Among 18 pairs of autosomes, first 5 pairs were sub-metacentric followed by 6th and 7th pair as sub-telocentric, next 5 pairs (8th to 12th pairs) were metacentric and remaining six pairs (13th to 18th) were telocentric. Among these one pair of allosomes i.e. X-chromosome was large metacentric, while Y-chromosome was metacentric and smallest chromosome of the karyotype. The least squares mean relative length of autosomes in LWY crossbred pigs ranged from 2.79±0.02 to 10.83±0.05 in females and 2.81±0.05 to 10.87±0.07 in males. The least squares mean relative length values for non-descript pigs ranged from 2.84±0.03 to 11.01±0.07 in females and 2.86±0.03 to 11.04±0.05 in males. The relative contribution of X-chromosome in LWY crossbred female and male pig was 4.67±0.01 and 4.65±0.04, whereas the values were 4.82±0.03 and 4.80±0.04 in non-descript pigs respectively. The mean relative contribution of Y-chromosome were 2.05±0.10 and 2.19±0.13 in LWY crossbred and non-descript pigs respectively. Genetic group had significant effect on the relative length of all autosomes and allosomes except 17th and 18th chromosomes, whereas sex had no significant effect on relative length of all chromosomes in both the breeds. The overall mean arm ratio of 12 pairs of autosomes ranged from 1.01±0.03 to 2.45±0.03 and 1.02±0.01 to 2.43±0.02 in LWY crossbred females and males respectively, whereas these values varied from 1.03±0.02 to 2.51±0.06 in females and 1.04±0.06 to 2.52±0.03 in males of non-descript pigs. The mean arm ratio values of X-chromosome was 1.15±0.04 in females and 1.13±0.03 in males of LWY crossbred pigs, while the respective mean values in non-descript pigs were 1.14±0.03 and 1.12±0.03 respectively. The arm ratio values of Y-chromosome were 1.17±0.02 and 1.18±0.02 in LWY crossbred and non-descript pigs. The mean centromeric indices of autosomes (1 to 12 pairs) differed from 0.50±0.04 to 0.71±0.02 and 0.52±0.03 to 0.72±0.03 in LWY crossbred female and male pigs, whereas it ranged from 0.51±0.01 to 0.82±0.01 and 0.53±0.05 to 0.83±0.02 respectively in non-descript pigs. The X-chromosome had mean centromeric index values of 0.53±0.03 and 0.52±0.03 in LWY crossbred female and male pigs, while the value for Y-chromosome was 0.56±0.02. The mean centromeric index values of X-chromosome in non-descript pigs were 0.52±0.02 and 0.54±0.02 in females and males, whereas for Y-chromosome, it was recorded as 0.55±0.01 respectively. The average morphological index value of autosomes ranged from 2.43±0.06 to 7.29±0.11 in females and 2.41±0.05 to 7.31±0.12 in males of LWY crossbred pigs, while X-chromosome had 5.85±0.05 and 5.84±0.05 respectively. The mean values for autosomes ranged in non-descript pigs were 2.42±0.02 to 7.96±0.14 and 2.44±0.04 to 7.98±0.13 in females and males, whereas X-chromosome had an index value 6.01±0.02 and 5.99±0.03 respectively. The values for Y-chromosome were 2.01±0.02 and 2.09±0.01 in LWY crossbred and non-descript pigs. In the present investigation with 128 metaphase spreads, a total of 311 NORs were detected in LWY crossbred and non-descript pigs. The mean number of NORs per metaphase was 2.67 and 2.20 in both breeds respectively. The highest frequency of metaphases (47.61% and 81.54%) had 2 number of NORs, whereas lowest frequency of metaphases (7.94% and 3.07%) had 4 number of NORs. The percentage share of silver deposits on 10th pair of chromosome accounts for 96.67 and 68.33 in LWY crossbred and non-descript pigs, whereas on 8th chromosome pair the percentage share of deposits were 44.16 and 51.67 respectively. The outcome of present study have revealed that the morphology, number and various morphometric measurements of the chromosomes in both the breeds were analogous to those reported in literature for various pig breeds like Canadian Lacombe, Landrace, Duroc, Thai wild boar, Nigerian indigenous pig, Niang Megha pig, Korean native pig, Polish Landrace, Hampshire, Large White Yorkshire, Turkish sus scrofa. Therefore, the efforts should be undertaken to recognize the non-descript pigs as a distinct breed of Andhra Pradesh. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810085008 | |
| dc.keywords | INDIGENOUS PIGS;CYTOGENETIC PROFILE;CROSSBRED | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 92 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA | en_US |
| dc.sub | Animal Genetics and Breeding | en_US |
| dc.subject | null | en_US |
| dc.theme | A COMPARATIVE STUDY ON CYTOGENETIC PROFILE OF CROSSBRED AND INDIGENOUS PIGS | en_US |
| dc.these.type | M.V.Sc. | |
| dc.title | A COMPARATIVE STUDY ON CYTOGENETIC PROFILE OF CROSSBRED AND INDIGENOUS PIGS | en_US |
| dc.type | Thesis | en_US |