IDENTIFICATION AND COMPARATIVE STUDIES OF WILD ISOLATES OF PINK OYSTER MUSHROOM
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Date
2023-12-19
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UHF, NAUNI
Abstract
The present investigation entitled “Identification and comparative studies of wild isolates of pink oyster mushroom” were undertaken to evaluate the macroscopic and microscopic identification, cultural requirements, cultivation, molecular characterization, nutritional analysis, mycochemical analysis and antioxidant properties of wild isolates. At macroscopic level, isolate P4 (commercially cultivated) exhibited largest size of pileus however, the stipe length was found to be maximum in wild isolate P3 whereas the largest size of basidiospores was observed in isolate P4. Irrespective of the isolates, hyphae were septate, branched with clamp connections. On PDA medium, maximum average diametric growth was shown by isolate P1 followed by isolate P4 and isolate P3, least mycelial growth was recorded in isolate P2. During the evaluation of different temperatures and pH regimes on mycelial growth it was observed that temperature 20-25℃ and pH 7.0 was optimum for all the isolates. All the selected isolates were screened for their fruiting behavior on wheat straw. Isolate P4 took less time for spawn run (17.75 days), primordial initiation (22.25 days) and recoded highest biological efficiency (73.92%) among wild isolates. Isolate P1 and P3 which showed fruiting on wheat straw were selected for molecular studies. Sequencing and sequence analysis using BLAST showed 96.80% and 95.27% similarity with the available sequence of Pleurotus djamor. Isolate P1 and P3 were assigned accession number ON202736 and OR491768 respectively. Nutritional analysis revealed that pink oyster mushrooms are bestowed with a wealth of nutrients including protein, carbohydrate, fibre content making them excellent superfood that enhance overall health. The moisture content (90.13%), protein content (26.60%), crude fibre (10.68%) was observed to be maximum in isolate P3. Mycochemical analysis revealed that pink oyster mushroom contains secondary metabolites such as phenols and flavonoids which have been regarded as the potent free-radical scavengers. Maximum content of phenols, flavonoids and antioxidant activity was recorded in wild isolate P3 indicating that their consumption can be beneficial in protecting the body from harmful free radicals which cause oxidative stress to the human body and reduce the risk of chronic diseases.