प्रवाह ससिंसित सिट्टी से पटें ाक्लोरोसिनोल जवै -रूपािंतरण जीवाणओु िं के लक्षण वणणन Characterization of Pentachlorophenol bio-transforming bacteria from effluent irrigated soil
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Date
2013
Authors
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Publisher
IARI,DIVISION OF MICROBIOLOGY
Abstract
The present study was carried out for isolation, identification and characterization of
pentachlorophenol biotransforming bacteria from pulp and paper mill effluent irrigated soils
of Lal Kuan, Uttrakhand. PCP is a high molecular weight, recalcitrant, hydrophobic aromatic
hydrocarbon compound toxic to all forms of life since it is an inhibitor of oxidative
phosphorylation. In solving the serious problems of pollution caused by PCP, it is important
to assess the potential of bacterial strains indigenous to sites contaminated with PCP for their
ability to degrade PCP.
Twenty two bacterial isolates were identified through 16S rDNA sequencing, which
grew well at 500 ppm of PCP as sole C source in minimal salt medium. However, reduction
in their growth was observed at 700 ppm PCP. Out of 22 isolates, 19 isolates belonged to
class - Proteobacteria, matched with sequences of Pseudomonas and Enterobacter. Two
isolates, Ochrobactrum sp (LK59) and Ensifer adhaerens (LK 4) belonged to α-
Proteobacteria. The single isolate of Firmicutes phyla was identified as Lysinibacillus
fusiformis (LK156) which could show significantly higher growth at 300 and 500 ppm of PCP
than other isolates. PCP degradation by Ensifer adhaerens and Lysinibacillus fusiformis is
reported for the first time in the present study and these might represent new chlorophenoldegrading
taxa.
HPLC studies revealed decrease in the residual PCP and formation of intermediates
such as tetrachlorohydroquinone (TeCH) and dichlorophenols (DCP) by selected isolates.
Removal of PCP from sterile soil by selected isolates, viz. Ensifer adhaerens, Pseudomonas
putida and Lysinibacillus fusiformis , was studied in a 30 d experiment under controlled
condition. These isolates were used alone and in combination. PCP removal by mixed
inoculum was significantly higher (68.81%) than single inoculation after 30 d of incubation.
The results of this study indicate that ability to degrade pentachlorophenol is widely
distributed amongst phylogenetically diverse group of bacteria in agricultural soils irrigated
with effluent discharged from pulp and paper mill. The enriched mixed inoculum has better
potential in scavenging PCP from soil as compared to individual isolates. The potential
isolates obtained in the present study can be used for developing consortium for
bioremediation of sites contaminated with PCP.
Description
T-8841
Keywords
grain, rice, planting, yields, developmental stages, genotypes, genetics, fertilizers, biological phenomena, phenotypes