TOXIN GENE PROFILING AND THERAPEUTIC STUDIES OF STAPHYLOCOCCUS AUREUS IN CAPRINE MASTITIS

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dc.contributor.advisorSMITHA J P
dc.contributor.authorMUKESHA KINI M A
dc.date.accessioned2020-07-24T05:13:48Z
dc.date.available2020-07-24T05:13:48Z
dc.date.issued2018
dc.description.abstractGoats are one of the most important livestock animals because of the high profitable margin and low investment which makes them suitable for small and marginal farmers. Loss of production of milk leads to undernourishment of kids and subsequently death. Hence it is important to control mastitis in goats. The present study was designed to know the etiology, occurrence of mastitis and the sensitivity pattern of the pathogens in Wayanad district. The study mainly concentrated on Staphylococcus species and that too S. aureus. A total of 45 milk samples were collected from goats suffering from clinical mastitis. The milk was subjected to culture and sensitivity tests. Out of the 45 isolates, S. aureus was the most predominant organism (33.3%) followed by coagulase negative staphylococcus (28.8%), E. coli, Klebsiella spp., Pseudomonas (8.8% each), Streptococcus spp., Enterobacter spp., (4.4% each) and S. intermedius (2.2%). The most sensitive antibiotic in vitro showed ceftriaxone-tazobactam as the most sensitive antibiotic (97.8%) followed by cefoperazone sulbactam (86.3%), cefoperazone (74%), tetracycline, enrofloxacin (60% each) and amoxycillin (57%). The goats suffering from staphylococcal mastitis were considered for the treatment trial. The test subjects were divided into three groups. The first group was treated with amoxycillinsulbactam, the second group with cefoperazone-sulbactam and the third with enrofloxacin. All the goats were treated for five days and in addition to antibiotics supportive therapy was given. The clinical cure and was assessed after five days. Molecular detection of virulence factor of S. aureus, namely staphylococcal enterotoxin (SEA, SEB, SEC, SED), Exfoliative toxin A (ETA), Toxic shock syndrome toxin-1 (TSST1), Staphylococcal protein A and accessory gene regulator 1 (AGR1) was done. Most of the S. aureus carried SPA (86.6%), only 40% carried AGR1 and SEA and SEB were detected in 13.3 per cent of isolates each. Rest of the toxins were not detected in any of the isolates.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810149742
dc.keywordsTOXIN GENE PROFILING THERAPEUTIC STUDIES STAPHYLOCOCCUS AUREUS ICAPRINE MASTITISen_US
dc.language.isoenen_US
dc.pages76en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANADen_US
dc.research.problemTOXIN GENE PROFILING AND THERAPEUTIC STUDIES OF STAPHYLOCOCCUS AUREUS IN CAPRINE MASTITISen_US
dc.subVeterinary Epidemiology and Preventive Medicineen_US
dc.subjectnullen_US
dc.themeTOXIN GENE PROFILING AND THERAPEUTIC STUDIES OF STAPHYLOCOCCUS AUREUS IN CAPRINE MASTITISen_US
dc.these.typeM.V.Sc.en_US
dc.titleTOXIN GENE PROFILING AND THERAPEUTIC STUDIES OF STAPHYLOCOCCUS AUREUS IN CAPRINE MASTITISen_US
dc.typeThesisen_US
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