EVALUATION OF EFFECT OF NUTRIENTS AND PLANT HORMONES AGAINST TOMATO LEAF CURL VIRUS AND SCREENING OF TOMATO BREEDING LINES FOR RESISTANCE
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Date
2018-08-01
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University of Agricultural Sciences, Bangalore
Abstract
Nutrients and plant hormones were evaluated against tomato leaf curl virus disease (ToLCVD). Among the plant nutrients sprayed a lowest disease incidence (20%) was recorded in the plants sprayed with the boron at inoculation period of 5 Days After Spray (DAS) and an increase in activity of defence enzymes like Peroxidase (PO), Poly Phenol Oxidase (PPO) and Phenyl Ammonia Lyase (PAL) (0.182, 2.40 and 13.76 μmol min-1 mg-1 protein, respectively) and higher amount of total phenols (2.07 mg/100mg), total proteins (1.27 mg/100mg) and chlorophyll content of 10.15 SPAD meter reading were recorded. Among the varied concentrations of Salycylic Acid (SA) applied, application of 250 μM of SA at inoculation period of 5 DAS showed a lower disease incidence (15%) and an increase in activity of PO, PPO and PAL (0.292, 2.99 and 18.36 μmol min-1 mg-1 protein, respectively) and higher amount of total phenols (2.17 mg/100mg), total proteins (1.27 mg/100mg) and chlorophyll content of 9.67 SPAD meter reading were recorded. Among the different concentrations of Naphthalene Acetic Acid (NAA) sprayed, spraying of NAA at 15 ppm at inoculation period of 5 DAS recorded a lowest disease incidence (30%) and an elevated levels of PO, PPO and PAL (0.192, 2.45 and 19.16 μmol min-1 mg-1 protein, respectively) and also higher amount of total phenols (2.15 mg/100mg), total proteins (1.17 mg/100mg) and chlorophyll content of 9.4 SPAD meter reading. Out of 62 F2 tomato genotypes and 10 hybrids screened for ToLCVD resistance, one F2 genotype (COHBTTF2/130-7) showed a resistant reaction.