Fingerprinting satawar (asparagus racemosus) genotypes using RAPD markers
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Date
2005
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Publisher
CCSHAU
Abstract
Satawar (Asparagus racemosus) is a medicinal plant growing in
tropical climates and is useful in curing a wide array of ailments.
This study was thus undertaken to prepare a DNA fingerprint
database of selected varieties of Asparagus racemosus and to assess
LITERATURE CITED -xivgenetic
diversity among them using randomly amplified polymorphic
DNA (RAPD) markers. Twenty five RAPD primers were used to assess
molecular polymorphism in fifteen Asparagus racemosus genotypes.
A total of 211 amplified products were obtained out of which 50 were
monomorphic and 161 were polymorphic. Average polymorphism
across fifteen genotypes was found out to be 78.650%. For the
genotypes tested, 5 to 17 bands were obtained, with an average of
10.55 bands per primer. The size of amplified fragments ranged from
230-2250 bp. Some primers also produced unique alleles in specific
Asparagus genotypes which could be used to distinguish them.
Analysis of this polymorphism profile, generated using suitable
statistical programmes, grouped the fifteen genotypes into two major
clusters at a similarity coefficient of 0.680. Varieties HAR-6 and
Wild-3 were found out to be the most diverse and distant from other
varieties. The second cluster again divided into two minor clusters,
out-grouping the Nepali variety. The next large sub-cluster
contained all the other genotypes. Varieties Wild-1 and Indian
Yellow were genetically most similar. Genetic similarity matrices of
the genotypes ranged from 0.630 to 1.00, indicating a moderate
genetic variability among the genotypes. Wild-1 and Indian Yellow,
showed a genetic similarity value of 1.00 while the genotypes HAR-1
and Wild-3 were found out to be genetically most diverse, at a value
of 0.630. All other genotypes varied between these two extreme
values. The results indicated that RAPD markers are efficient for
identification of Asparagus racemosus genotypes and for
determination of the genetic relationships among them. Fingerprint
data obtained in this study can be further utilized in identification
and development of improved Asparagus varieties.