Anti-Bacterial Activity Of Silver Nanoparticles Synthesized using Phyllanthus Amarus Aqueous & Andrographis Paniculata Ethanolic Extracts
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Date
2015-01
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TANUVAS, Chennai
Abstract
The use of metallic silver as an antimicrobial agent has been recognized
for centuries. Silver nanoparticles (SNP) are now incorporated in apparel,
wound dressings, appliances, cosmetics, paints and plastics for their
antimicrobial properties. Generally, silver nanoparticles are prepared by a
variety of chemical methods. In this study, we have used Phyllanthus amarus
and Andrographis paniculata herbal plants leaves and stem extracts to synthesis
SNP and were characterized by UV-Vis spectroscopy and Transmission Electron
Microscopy (TEM). SNP capped with plant extracts gave absorption peak at
420 nm as expected for silver and broadening of peak were also noticed. TEM
images suggested that they were of almost spherical shape and in the range of
7-60 nm in size. Antibacterial activity of plant extracts capped SNP were tested
in Mueller-Hinton agar by well diffusion method against Staphylococcus
aureus, Bacillus subtilis, Proteus vulgaris, Escherichia coli and Salmonella
Typhimurium. SNP of 1 mg/ml and 5 mg/ml of P.amarus exhibited almost
similar antibacterial activity, whereas, 5mg/ml, not 1 mg/ml of SNP capped
with A.paniculata exerted antibacterial activity. The highest zone of inhibition
was noticed against B.subtilis followed by S.aureus and P.vulgaris and the
least zone of inhibition was observed against E.coli and S.Typhimurium. Green
synthesis of SNP is cost effective and environment friendly. Further studies
are required to explore the possibility of use of P.amarus capped silver
nanoparticles for the treatment of burn and wound.
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Veterinary Science