FREEZABILITY OF BOAR SEMEN SUPPLEMENTED WITH SODIUM DODECYL SULPHATE IN LOW OR HIGH SPERM CONCENTRATION DOSES

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dc.contributor.advisorHiron M. Harshan
dc.contributor.authorMEGHA WILSON
dc.date.accessioned2020-07-23T08:31:54Z
dc.date.available2020-07-23T08:31:54Z
dc.date.issued2018
dc.description.abstractBoar semen has conventionally been frozen with egg yolk based extenders containing Orves Es Paste (OEP). Sodium dodecyl sulphate (SDS) is the major component of OEP, which interacts with egg yolk lipids for improving their availability for spermatozoa. In case of boar semen, high concentration packing has been preferred as post-thaw damage are low with it. The objective of this study was to compare the post-thaw attributes of boar semen, when extended with extender supplemented with and without SDS and packed at high and low sperm concentration. Four ejaculates each were collected and cryopreserved from four adult healthy Large White Yorkshire boars (total 16 ejaculates). The fresh semen was evaluated for its volume, colour, pH, sperm concentration, sperm progressive motility, sperm viability, sperm abnormality, sperm acrosome integrity and hypo osmotic sperm swelling (HOS) response. The collected ejaculate was extended 1:1 extended which was then extended with lactose egg yolk extender (LEY) to either contain 1500 over 90 min and further extended with LEY with glycerol in such a way as to form four groups, namely group I (1000 × 106 sperm/mL), group II (1000 × 106 sperm/mL with 0.2% SDS), group III (200 × 106 sperm/mL) and group IV (200 × 106 sperm/mL with 0.2% SDS). The pre-freeze sperm motility values did not show any significant difference between boars or treatment. Pre-freeze sperm viability, abnormality, acrosome integrity, HOS response differed significantly between the boars (p<0.01), but did not differ between the groups. Post-thaw sperm attributes, except for sperm abnormalities, showed better values in groups supplemented with SDS. Better values were also observed for these post-thaw traits when semen was packaged at higher concentration. Incorporation of SDS or varying sperm concentration in packing did not influence post-thaw abnormal sperm counts. The results revealed that, there is an improvement of post-thaw boar sperm characteristics with supplementation of SDS into egg yolk based extender and with high sperm concentration packing.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810149709
dc.keywordsFREEZABILITY BOAR SEMEN SUPPLEMENTED SODIUM DODECYL SULPHATE IN LOW HIGH SPERM CONCENTRATION DOSESen_US
dc.language.isoenen_US
dc.pages76en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANADen_US
dc.research.problemFREEZABILITY OF BOAR SEMEN SUPPLEMENTED WITH SODIUM DODECYL SULPHATE IN LOW OR HIGH SPERM CONCENTRATION DOSESen_US
dc.subAnimal Reproduction, Gynaecology and Obstetricsen_US
dc.subjectnullen_US
dc.themeFREEZABILITY OF BOAR SEMEN SUPPLEMENTED WITH SODIUM DODECYL SULPHATE IN LOW OR HIGH SPERM CONCENTRATION DOSESen_US
dc.these.typeM.V.Sc.en_US
dc.titleFREEZABILITY OF BOAR SEMEN SUPPLEMENTED WITH SODIUM DODECYL SULPHATE IN LOW OR HIGH SPERM CONCENTRATION DOSESen_US
dc.typeThesisen_US
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