FREEZABILITY OF BOAR SEMEN SUPPLEMENTED WITH SODIUM DODECYL SULPHATE IN LOW OR HIGH SPERM CONCENTRATION DOSES
Loading...
Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD
Abstract
Boar semen has conventionally been frozen with egg yolk based extenders containing
Orves Es Paste (OEP). Sodium dodecyl sulphate (SDS) is the major component of OEP,
which interacts with egg yolk lipids for improving their availability for spermatozoa. In
case of boar semen, high concentration packing has been preferred as post-thaw damage
are low with it. The objective of this study was to compare the post-thaw attributes of
boar semen, when extended with extender supplemented with and without SDS and
packed at high and low sperm concentration. Four ejaculates each were collected and
cryopreserved from four adult healthy Large White Yorkshire boars (total 16 ejaculates).
The fresh semen was evaluated for its volume, colour, pH, sperm concentration, sperm
progressive motility, sperm viability, sperm abnormality, sperm acrosome integrity and
hypo osmotic sperm swelling (HOS) response. The collected ejaculate was extended 1:1
extended
which was then extended with lactose egg yolk extender (LEY) to either contain 1500
over 90 min and further extended with LEY with glycerol in such a way as to form four
groups, namely group I (1000 × 106 sperm/mL), group II (1000 × 106 sperm/mL with
0.2% SDS), group III (200 × 106 sperm/mL) and group IV (200 × 106 sperm/mL with
0.2% SDS). The pre-freeze sperm motility values did not show any significant difference
between boars or treatment. Pre-freeze sperm viability, abnormality, acrosome integrity,
HOS response differed significantly between the boars (p<0.01), but did not differ
between the groups. Post-thaw sperm attributes, except for sperm abnormalities, showed
better values in groups supplemented with SDS. Better values were also observed for
these post-thaw traits when semen was packaged at higher concentration. Incorporation of
SDS or varying sperm concentration in packing did not influence post-thaw abnormal
sperm counts. The results revealed that, there is an improvement of post-thaw boar sperm
characteristics with supplementation of SDS into egg yolk based extender and with high
sperm concentration packing.
Description
Keywords
null