BIOCHEMICAL EVALUATION OF PHYTOSYNTHESIZED SILVER NANOPARTICLE INDUCED DEFENSE AGAINST PEST, TIKKA DISEASE AND CELLULAR STRESS RESPONSE IN GROUNDNUT CROP
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Date
2019-07-25
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE
Abstract
Nano science research has extensive applicability as nanoparticles have special
features and can also be exploited in the field of Agriculture. Earlier in our lab, Silver
nanoparticles (Ag NPs) have been phytosynthesized using Sandalwood leaf extract and
characterized by SEM, FTIR and XRD analysis. In this study, the applicability of the
phytosynthesized Ag NPs was tested on groundnut crop and a field experiment was taken
up with the variety GKVK 13 in Kharif of 2018 at GKVK, Bangalore. The study reveals
that Ag NP treatments significantly enhanced plant biomass and groundnut yield. The Ag
NP treatments also resulted in significant suppression of late leaf spot disease severity in
groundnut. The study demonstrates Ag NP induced defense priming-like response with
elevation of defense enzymes (SOD, POX) and secondary metabolites such as phenolics
and flavonoids in groundnut. The field experiments revealed higher pod yield (29.91%),
seed yield (47.18%) and shelling per cent (25%) as compared to control. The soil from the
Ag NP treated experimental plots did not show significant variation in terms of bacterial
and actinomycetes populations. The changes in membrane potential in groundnut calli
cells, exposed to Ag NPs, demonstrates an Ag NP induced depolarization that required
extracellular Ca2+
. The phytosynthesized Ag NP showed significant inhibitory effect (LC50
986.98 ppm) against the insect pest Diamond Back Moth. The study thus demonstrates that
phytosynthesized Ag NP has the potential of being used as a growth promoter and for the
management of phytopathogens and pests in crops.
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