VALIDATION OF A SIMPLE ISOCRATIC HPLC ASSAY FOR THE DETECTION OF OXYTETRACYCLINE RESIDUES IN CHICKEN MEAT

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dc.contributor.authorLavanya, G.
dc.contributor.authorRamesh, S.
dc.contributor.authorRamasamy, T.
dc.contributor.authorSangilimadan, K.
dc.contributor.authorKalaiselvi, L.
dc.contributor.authorTANUVAS
dc.date.accessioned2022-07-13T09:49:13Z
dc.date.available2022-07-13T09:49:13Z
dc.date.issued2022-01
dc.descriptionTNV_IJVASR_51(1)_Jan-Feb2022_12-20en_US
dc.description.abstractOne of the major concerns in poultry rearing has been the use of antibacterials for the control of infections. The antibacterial drugs often find their way into the edible part of chicken, thus making it an issue of human food safety. Screening of chicken meat for antibacterials is thus an important part of ensuring food safety. Simple sensitive methods which could be adopted easily at laboratories of research institutes and testing centres are a need of the hour. In this study, one such assay method for the assay of oxytetracycline, a popularly used antibiotic in poultry farms, in broiler chicken meat standardized using High-performance liquid chromatography (HPLC) is described. The HPLC assay method consisted of a C18column as the stationary phase and a mobile phase consisting of 0.2M oxalic acid: Acetonitrile: Methanol (3.5:1:1, v/v) pumped in with the flow rate of 1.5 ml/ min and assayed at 360nm for detection of OTC. The Limit of Detection and Limit of Quantification was 0.141 μg/ ml and 0.465 μg/ ml respectively. The total elution time was less than 4 minutes with a recovery rate of 105%. The results suggest that this method, with its high detection throughput and remarkable sensitivity and specificity, can be applied successfully for the field screening of oxytetracycline residue in chicken meat samples.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810185430
dc.keywordsVeterinary Science, Veterinary Pharmacology and Toxicology, Broiler chicken meat, HPLC LOD and LOQ, Oxytetracycline Residue, Recovery, Repeatabilityen_US
dc.language.isoEnglishen_US
dc.pages12-20en_US
dc.publisherTANUVAS, Chennaien_US
dc.relation.ispartofseries;1
dc.subjectVeterinary Scienceen_US
dc.subjectVeterinary Pharmacology & Toxicologyen_US
dc.titleVALIDATION OF A SIMPLE ISOCRATIC HPLC ASSAY FOR THE DETECTION OF OXYTETRACYCLINE RESIDUES IN CHICKEN MEATen_US
dc.title.alternativeInd. J. Vet. & Anim. Sci. Res.en_US
dc.typeArticleen_US
dc.volume51en_US
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