SYNTHESIS OF NANOPARTICLE FROM BIORESOURCES FOR MANAGEMENT OF BLAST DISEASES OF RICE

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dc.contributor.advisorBhattacharyya, Ashok
dc.contributor.authorKaman, Pranjal Kumar
dc.date.accessioned2023-12-13T16:17:48Z
dc.date.available2023-12-13T16:17:48Z
dc.date.issued2021
dc.description.abstractDevelopment of reliable and eco-friendly process for synthesis of metallic nanoparticle is an important steps in the field of nanotechnology. An effort was made for biosynthesis of gold nanoparticles from bioresources like microbes Aspergillus niger, Metarhizium anisopliae, Lecanicillium lecannii, Purpeurocillium lilacinus and botanicals like Ocimum gratissimum, Acorus calamus, Aloe barbadensis and Azadirachta indica. Chloroauric acid was added as precursor for the synthesis of gold nanoparticles. Study on in vitro efficacy of biosynthesized gold nanoparticle from bioresources was tested at hundred percent concentration against Magnaporthe grisea and effective source was found to be Metarhizium anisopliae and Ocimum gratissimum. Further efficacy of biosynthesized gold nanoparticle from Metarhizium anisopliae and Ocimum gratissimum. was tested against Magnaporthe grisea at three different concentrations (50, 100 and 150ppm) comparison was made with Tryclozole @ 600 ppm. The result showed that the gold nanoparticles at 150 ppm significantly inhibit the mycelia growth of the pathogens as compared to the Tryclozole @ 600 ppm. The effective biosynthesized gold nanoparticles was characterized by UV-Vis spectrophotometer, Dynamic Light Scattering (DLS), Fourier transform infrared spectrometer (FTIR), Zeta Sizer and Transmission Electron Microscope (TEM). Formation of gold nanoparticles were confirmed by UV-VIS spectroscopy study with absorption peaks at 550 nm. FTIR study showed that synthesized gold nanoparticle has all the required functional groups like OH, N-H, C-H and COO-. Study on surface properties of nanoparticles by using zetasizer resulted that gold nanoparticle from Metarhizium anisopliae was found to be negative and were stable in nature with zeta potential value of -20.7 mV. DLS analysis showed that the average size of the biosynthesized gold nanoparticles is 32.54 nm with polydispersity index of 0.560. TEM study showed that shape of the biosynthesized nanoparticle is from triangular to quasihedral and the size range from 9-54nm. Again, a pot experiment was conducted for studying its effect on morphophysiology as well as soil physico chemical and biological properties by different methods of application like seedling dip treatment, foliar spray and soil application. A positive effect was found on the morphology, soil physico chemical and biological properties when rice seedling was treated as seedling dip treatmen + foliar spray + Soil application @ 150ppm.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810202860
dc.language.isoEnglish
dc.subPlant Pathology
dc.themeSYNTHESIS OF NANOPARTICLE FROM BIORESOURCES FOR MANAGEMENT OF BLAST DISEASES OF RICE
dc.these.typePh.D
dc.titleSYNTHESIS OF NANOPARTICLE FROM BIORESOURCES FOR MANAGEMENT OF BLAST DISEASES OF RICE
dc.typeThesis
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