SYNTHESIS OF NANOPARTICLE FROM BIORESOURCES FOR MANAGEMENT OF BLAST DISEASES OF RICE
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Date
2021
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Abstract
Development of reliable and eco-friendly process for synthesis of
metallic nanoparticle is an important steps in the field of nanotechnology. An effort was
made for biosynthesis of gold nanoparticles from bioresources like microbes Aspergillus
niger, Metarhizium anisopliae, Lecanicillium lecannii, Purpeurocillium lilacinus and
botanicals like Ocimum gratissimum, Acorus calamus, Aloe barbadensis and Azadirachta
indica. Chloroauric acid was added as precursor for the synthesis of gold nanoparticles.
Study on in vitro efficacy of biosynthesized gold nanoparticle from bioresources was
tested at hundred percent concentration against Magnaporthe grisea and effective
source was found to be Metarhizium anisopliae and Ocimum gratissimum. Further efficacy of
biosynthesized gold nanoparticle from Metarhizium anisopliae and Ocimum gratissimum. was
tested against Magnaporthe grisea at three different concentrations (50, 100 and 150ppm)
comparison was made with Tryclozole @ 600 ppm. The result showed that the gold
nanoparticles at 150 ppm significantly inhibit the mycelia growth of the pathogens as
compared to the Tryclozole @ 600 ppm. The effective biosynthesized gold
nanoparticles was characterized by UV-Vis spectrophotometer, Dynamic Light
Scattering (DLS), Fourier transform infrared spectrometer (FTIR), Zeta Sizer and
Transmission Electron Microscope (TEM). Formation of gold nanoparticles were
confirmed by UV-VIS spectroscopy study with absorption peaks at 550 nm. FTIR study
showed that synthesized gold nanoparticle has all the required functional groups like
OH, N-H, C-H and COO-. Study on surface properties of nanoparticles by using
zetasizer resulted that gold nanoparticle from Metarhizium anisopliae was found to be
negative and were stable in nature with zeta potential value of -20.7 mV. DLS analysis
showed that the average size of the biosynthesized gold nanoparticles is 32.54 nm with
polydispersity index of 0.560. TEM study showed that shape of the biosynthesized
nanoparticle is from triangular to quasihedral and the size range from 9-54nm. Again, a
pot experiment was conducted for studying its effect on morphophysiology as well as
soil physico chemical and biological properties by different methods of application like
seedling dip treatment, foliar spray and soil application. A positive effect was found on
the morphology, soil physico chemical and biological properties when rice seedling was
treated as seedling dip treatmen + foliar spray + Soil application @ 150ppm.