Phytochemical characterization and evaluation of different biological activities of Mosla dianthera (Buch.-Ham. ex Roxb.) Maxim. and Plectranthus barbatus Andrews

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dc.contributor.advisorOm Prakash
dc.contributor.authorKanyal, Jeewanti
dc.date.accessioned2021-11-18T04:31:48Z
dc.date.available2021-11-18T04:31:48Z
dc.date.issued2020-02
dc.description.abstractIn present study the chemical composition by GC/MS, isolation of the compounds by column chromatography, characterization of isolated compounds by spectroscopic techniques, biochemical assay and their different biological activities like antioxidant, in vitro antiinflammatory, herbicidal, antibacterial, antifungal and antifeedant activities of essential oils, various extracts and isolated compounds from Mosla dianthera and Plectranthus barbatus were evaluated. The yields of essential oils and extracts from M. dianthera and P. barbatus were observed 0.1-0.4% and 1.3-12.0% respectively. Carvone, n-decanal, (-)-bornyl acetate, capric aldehyde, thymol, p-cymene, β-pinene and α-pinene were identified as major components in essential oils. Cellobiose, forskolin, tetrapentacontane, thymoquinol, palmitic acid, γ-sitosterol, 2-ethyl-5-isopentylthiophene, 5-hydroxymethylfurfural and (Z)-9-octadecenal were identified as major components in extracts. The isolation of the compounds from aerial part essential oil of M. dianthera and root part ethyl acetate extract (polar fraction) of P. barbatus by column chromatography and characterization by FT-IR and NMR proofed the presence of carvone and forskolin respectively. The total phenolics, flavonoids and orthodihydric phenols in the extracts were found to vary from 13.72 to 47.72 mg/g gallic acid equivalent, 10.65 to 71.73 mg/g catechin equivalent and 4.55 to 10.27 mg/g catechol equivalent respectively. The antioxidant assay was evaluated by six different methods. The essential oils, extracts and isolated compounds exhibited antioxidant potential in a dose dependent manner. The in-vitro antiinflammatory activity of essential oils, extracts and isolated compounds was evaluated by using protein (albumin) denaturation method. A remarkable anti-inflammatory potential was exhibited by isolated compound forskolin (89.40%) and aerial part chloroform extract of M. dianthera (88.03%). The herbicidal activity was determined in term of inhibition of seed (radish) germination. All the essential oils, extracts and isolated compounds showed moderate to strong herbicidal potential in a dose dependent manner. The essential oils, extracts and isolated compounds exhibited significant antibacterial activity against two pathogenic bacteria. Among all, isolated compound forskolin was found to most effective against B. cereus and E. coli. Similarly, the antifungal activity of essential oils, extracts and isolated compounds was tested against two phytopathogenic fungi and express in term of inhibition of mycelial growth of fungi. The essential oils were found more effective for inhibiting the growth of fungus. Among all, aerial part essential oil of M. dianthera exhibited maximum inhibition (100%) of mycelial growth of A. alternata and C. lunata at higher concentration (500 ppm). The antifeedant activity was evaluated by using leaf disc method in no-choice situation. All the essential oils, extracts and isolated compounds exhibited a diverse category of antifeedant activity (moderate to extreme antifeedant). Among all, the maximum antifeedant activity was observed in aerial part essential oil of M. dianthera (90.70%), followed by isolated compounds carvone (89.17%) and forskolin (87.60%) against S. obliqua (Bihar hairy caterpillar) larvae.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810178149
dc.keywordsphytochemistry, characterization, biological activity, Mosla dianthera, Plectranthus barbatusen_US
dc.language.isoEnglishen_US
dc.pages437en_US
dc.publisherG.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)en_US
dc.research.problemPlant Extractsen_US
dc.subChemistryen_US
dc.themePhytochemistryen_US
dc.these.typePh.Den_US
dc.titlePhytochemical characterization and evaluation of different biological activities of Mosla dianthera (Buch.-Ham. ex Roxb.) Maxim. and Plectranthus barbatus Andrewsen_US
dc.typeThesisen_US
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