Molecular marker analysis for QTL identification in Indian mustard (Brassica juncea L. Czern & Coss.) for salinity tolerance
| dc.contributor.advisor | Yadav, Neelam R. | |
| dc.contributor.author | Patel, Rekha | |
| dc.date.accessioned | 2017-09-22T04:46:18Z | |
| dc.date.available | 2017-09-22T04:46:18Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | Salinity tolerance is quantitative and complex phenomenon controlled by numerous interacting quantitative trait loci (QTLs). To find out genomic regions associated with terminal salinity tolerance a study entitled Molecular marker analysis for QTL identification in Indian mustard (Brassica juncea L. Czern & Coss.) for salinity tolerance was conducted with the objective to identify QTLs for salinity tolerance in RH 30 × CS 52 derived F2 and F2:3 populations of Brassica juncea (RH 30-Salinity sensitive and CS 52-Salinity tolerant) and to evaluate both generations for yield attributes such as plant height (cm), physiological maturity, main shoot length (cm), number of primary branches/plant, number of secondary branches/plant, number of siliquae/plant, number of seeds/siliqua, 1000-seed weight (g), seed yield/plant (g) and biological yield/plant (g); physiological traits such as relative water content (%), osmotic potential (-MPa), proline content (µmol/g) and membrane injury (%) under field and salinity treatments i.e. control, 6 dS/m and 8 dS/m. Out of 358 SSR markers 42 were found polymorphic and 153 were monomorphic. Linkage map was prepared using 41 SSR markers covering 8 linkage groups and 1 marker did not map to any of the linkage groups. A total of 91 alleles were identified in the CS 52 and RH 30 genotypes. The total length of the genetic linkage map was 2298.5 cM and PIC ranged from 0.427-0.730. Composite interval mapping revealed a total of 23 QTLs were identified for yield attributes and physiological traits in F2 population. Five QTLs identified for salinity tolerance on two linkage groups 4 and 8 under field and salinity treatments. QTLs identified in the present study firstly needs to be validated in other populations and then fine mapping of these salinity related QTLs have to be carried with them in marker assisted selection and breeding for salinity tolerant genotype in Brassica juncea. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810031671 | |
| dc.keywords | Brassica juncea, Quantitative trait loci, Salinity tolerance, Linkagemap, SSR markers | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | CCSHAU | en_US |
| dc.sub | Biotechnology and Molecular Biology | |
| dc.subject | null | en_US |
| dc.theme | Molecular marker analysis for QTL identification in Indian mustard (Brassica juncea L. Czern & Coss.) for salinity tolerance | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | Molecular marker analysis for QTL identification in Indian mustard (Brassica juncea L. Czern & Coss.) for salinity tolerance | en_US |
| dc.type | Thesis | en_US |