STUDIES ON THE EFFECT OF CANNABIS SATIVA AGAINST DATURA STRAMONIUM- INDUCED TOXICITY IN RATS
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Date
2016-12-14
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CSKHPKV, Palampur
Abstract
The present investigation was undertaken to determine the efficacy of Cannabis sativa against Datura stramonium-induced toxicity in rats. A total of 72 (36 male and 36 female) weaned Wistar rats were randomly divided into six groups viz. Group-I served as normal control and received vehicle (NSS) only; Group-II rats treated with D. stramonium leaf extract @ 2000 mg/kg bw; Group-III rats treated with C. sativa root extract @ 1200 mg/kg bw; Group-IV rats treated with D. stramonium leaf extract @ 2000 mg/kg bw plus C. sativa root extract @ 400mg/kg bw; Group-V rats treated with D. stramonium leaf extract @ 2000 mg/kg bw plus C. sativa root extract @ 800 mg/kg bw; and Group-VI rats treated with D. stramonium leaf extract @ 2000 mg/kg bw plus C. sativa root extract @ 1200 mg/kg bw for 28 days by oral route. D. stramonium produced significant toxicity in rats from the first week of experimentation as seen by the clinical signs including typical nervous signs, mortality among 2 males and the significant reduction in the body weights on 14th and 21st day onwards in male and female rats, respectively. The absolute weights of kidneys, spleen and gonads were significantly decreased in female rats; however, relative organ weights of liver, kidneys, heart, brain and gonads were significantly increased in male rats. Haematological changes included significantly decreased PCV and MCHC and an increased MCV values seen in male rats. The serum activities such as alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, serum enzymatic creatinine were significantly increased while, the decreased total protein levels and increased globulin and A:G ratio were noted in male and female rats. Gross pathological changes were less marked in group-II. However, histopathological changes were severe and consistently seen in Group-II rats with toxico-pathological damage in all the internal organs such as liver, kidneys, heart, brain, spleen, small intestine, mesenteric lymph nodes and gonads. No toxico-pathological changes were seen in the male and female rats given Cannabis sativa (Group-III) throughout the study. However, simultaneous administration of Cannabis to male and female rats from Groups-IV, V and VI was effective in alleviating the D. stramonium-induced toxicity in a dose-dependent manner.
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