MOLECULAR DETECTION AND HISTOPATHOLOGICAL STUDIES OF VIRAL DISEASES AFFECTING NERVOUS SYSTEM OF DOGS AND CATS

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dc.contributor.advisorPrasanna K. S.
dc.contributor.authorSHYAM S
dc.date.accessioned2020-07-24T05:17:06Z
dc.date.available2020-07-24T05:17:06Z
dc.date.issued2018
dc.description.abstractA number of viruses affects the nervous system of dogs and cats resulting in various diseases. The common viruses affecting nervous system of dogs include Rabies virus (RV), Canine Distemper virus (CDV) and Suid herpes virus-1 (SHV-1) and that of cats are Feline Panleukopenia virus (FPV) and Feline Infectious Peritonitis virus (FIPV). A comprehensive study was done to detect the presence of RV, CDV, SHV-1, FPV and FIPV in dogs and cats in Kerala. A total of 67 brain tissue samples collected from 67 dogs and cats of various districts were utilized for the study. The tissue samples were examined for gross lesions as well as histopathological lesions after formalin fixation and further tissue processing. Lesions like negri body, Babes nodules, perivascular cuffing, hypoplasia of the pyramidal cell layer, necrosis of the purkinje cells were the important histopathological lesions found in brain tissues examined in different positive cases. Molecular detection was also carried out after RNA/ DNA extraction. For RV detection, direct Fluorescent Antibody Test (dFAT) and reverse transcription polymerase chain reaction (RT-PCR) targeting N gene region was carried out and 15 samples were detected as positive. By sequencing and BLAST analysis, it was found that RV isolate has similarity with Indian isolates. For CDV detection, RT-PCR targeting N gene region were done and two samples were detected as positive. On BLAST analysis, it was found that CDV isolates had much similarity to other Indian isolates. For FPV detection, PCR targeting VP2 gene was carried out and eight samples were detected as positive. On BLAST analysis, it showed much similarity to Indian isolates. For SHV-1 detection, PCR targeting gB gene and for FIPV detection nested RT-PCR targeting 3’ UTR gene was carried out and none of the samples were detected as positive. The study could successfully detect the presence of rabies, canine distemper and feline panleukopenia in dogs and cats in various districts of north Kerala.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810149743
dc.keywordsMOLECULAR DETECTION HISTOPATHOLOGICAL STUDIES VIRAL DISEASES AFFECTING NERVOUS SYSTEM DOGS CATSen_US
dc.language.isoenen_US
dc.pages78en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANADen_US
dc.research.problemMOLECULAR DETECTION AND HISTOPATHOLOGICAL STUDIES OF VIRAL DISEASES AFFECTING NERVOUS SYSTEM OF DOGS AND CATSen_US
dc.subVeterinary Pathologyen_US
dc.subjectnullen_US
dc.themeMOLECULAR DETECTION AND HISTOPATHOLOGICAL STUDIES OF VIRAL DISEASES AFFECTING NERVOUS SYSTEM OF DOGS AND CATSen_US
dc.these.typeM.V.Sc.en_US
dc.titleMOLECULAR DETECTION AND HISTOPATHOLOGICAL STUDIES OF VIRAL DISEASES AFFECTING NERVOUS SYSTEM OF DOGS AND CATSen_US
dc.typeThesisen_US
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