MOLECULAR CHARACTERIZATION OF DIFFERENT CHLAMYDIAE ASSOCIATED WITH VARIOUS INFECTIONS OF RUMINANTS
| dc.contributor.advisor | Chahota, Rajesh | |
| dc.contributor.author | Bhardwaj, Brijesh | |
| dc.date.accessioned | 2019-02-02T07:04:27Z | |
| dc.date.available | 2019-02-02T07:04:27Z | |
| dc.date.issued | 2011-07 | |
| dc.description.abstract | Chlamydial infections have been reported previously among domestic ruminants from various disorders affecting reproductive system, respiratory system and digestive system in Himachal Pradesh, but the molecular characterization of various species/strains of chlamydiae involved has not been done yet, hence we conducted a molecular epidemiological investigation to detect the prevalence of different chlamydial species/strains in domestic ruminants in different parts of state, migratory tracts and in organized farms of Himachal Pradesh. To know the overall chlamydial prevalence in different disease conditions, total 404 samples were screened using Chlamydiales order specific primers based on 23S rRNA and family Chlamydiaceae specific primers based on VD2 region of ompA gene. Result of chlamydial screening showed that 93 (23%) samples, out of 404 samples were positive for chlamydiae, which showed the higher involvement of chlamydiae in various disease conditions. Out of 205 samples of reproductive disorders, 55 (26.82%) samples were showed chlamydial involvement. Among the cases of reproductive disorders, highest i.e. 38 per cent chlamydial association was detected in endometritis followed by 23 per cent in abortions. Out of 147 samples of pneumonia, 23 (15.64%) samples were found positive for chlamydial infection and from 39 samples of enteritis, chlamydial involvement was found in 33.33 per cent samples. Whereas, two samples of conjunctivitis were also found positive for chlamydial involvement. Molecular characterization and genetic variability studies of chlamydial species/strains detected among ruminants by PCR were done either by PCR-RFLP or by study of nucleotide sequence variation of ompA gene in VD2 region. Overall 57 PCR positive samples were characterized and out of which 39 (68.4%) samples were found C. psittaci, 16 (28%) samples were C. abortus, and two (3.5%) samples were found C. pecorum. From PCR positive samples, isolation of chlamydial strains was also attempted using 6 to 8 day old embryonated chicken eggs and isolation of chlamydiae up to 40.74 per cent could be achieved. Besides chlamydiae, involvement of other bacterial agents was also tested in samples from reproductive diseases. Various bacterial isolates like Brucella melitensis 2 (1.1%), Staphylococcus spp. 48 (26.37%), Streptococcus spp. 7 (3.84%), E. coli 47 (25.82%), Bacillus spp. 31 (17.03%), Klebsiella spp. 11 (6.04%), Arcanobacterium spp. 18 (10%), Pseudomonas spp. 13 (7.14%) and other bacterial species. 24 (13.19%) were isolated from different female reproductive disorders of the livestock. The results showed high prevalence of chlamydial infection among ruminants and involvement of multiple chlamydial species was detected in this study. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810093366 | |
| dc.keywords | Ruminants | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 80 | en_US |
| dc.publisher | CSKHPKV, Palampur | en_US |
| dc.sub | Veterinary Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | Molecular characterization of different chlamydiae | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | MOLECULAR CHARACTERIZATION OF DIFFERENT CHLAMYDIAE ASSOCIATED WITH VARIOUS INFECTIONS OF RUMINANTS | en_US |
| dc.type | Thesis | en_US |