Genetic trends in combining ability, heterosis and protein profiling in vegetable pea (Pisum sativum L.)
| dc.contributor.advisor | Singh, Y.V. | |
| dc.contributor.author | Bisht, Bhawana | |
| dc.date.accessioned | 2016-08-27T14:24:07Z | |
| dc.date.available | 2016-08-27T14:24:07Z | |
| dc.date.issued | 2008-05 | |
| dc.description | Thesis-PhD | en_US |
| dc.description.abstract | Present investigation was carried out during rabi season 2005-06 and 2006-07 at the Vegetable Research Centre of GBPUA&T, Pantnagar with 59 genotypes including 14 lines, 3 testers and 42 F1s of vegetable pea. The experiment was laid out in randomized block design with three replications. The observations were recorded for 15 characters viz., days to first flowering, number of first flowering node, days to first green pod picking, pod length (cm), 100 green pod weight (g), number of seeds per pod, 100 green seed wt. (g), shelling (%), T.S.S. (%), number of green pods per plant, number of primary branches per plant, number of nodes per main stem, plant height (cm), green pod yield per plant and dry seed yield per plant (g).The data were subjected to appropriate statistical analysis. Further, seed protein of 17 parents was utilized for sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The analysis of variance revealed highly significant differences among genotypes for all the attributes except two characters viz., days to first green pod picking and shelling percentage. High heritability coupled with high genetic advance as per cent of mean was recorded for Days to first flowering, number of first flowering node and green pod yield per plant indicating importance of additive gene action controlling these characters. The findings of present investigation revealed that PMR-53 (days to first green pod picking, pod length, 100-green pod weight, 100-green seed weight, number of primary branches per plant, number of nodes per main stem, green pod yield per plant (g) and dry seed yield per plant (g). PSM-3 (number of seeds per pod and dry seed yield per plant), E-6 (early maturity), VL-7 (number of seeds per pod), PSM-4 (days to first green pod picking, 100-green pod weight, number of green pods per plant, number of primary branches per plant and number of nodes per main stem), Arkel (number of seeds per pod and number of green pod per plant) and VP-266 (early maturity and T.S.S. (%)) were best general combiners. The lines PMR-53, PSM-3, E-6, VL-7, PSM-4, Arkel and VP-266 can be used as parents in the hybridization programme to get desirable recombinants in segregating generations. The cross Arka Ajit × Arkel could be exploited for early maturity. The crosses VRP-16 × VL-7, PMR-19 × PSM-3, Arka Ajit × PSM-3 and PSM-4 × Arkel showed maximum sca effects for most of the traits studied including the yield and yield attributing characters. Thus, these crosses could be advanced to recover desirable segregants for the improvement of yield and yield contributing characters. On the basis of standard heterosis, it can be concluded that the heterosis breeding would be advantageous for the improvement of pea for yield and its component quantitative traits. The crosses PMR-53 × PSM-3, E-6 × VL-7, PSM-4 × PSM-3, PSM-4 × Arkel, PMR-53 × VL-7 and VP-266 × PSM-3 could be exploited as commercial hybrids as they demonstrated highly significant heterosis, over the standard cultivar, Arkel and Arka Ajit. The seventeen genotypes used as parents in present study could be distinguished based on sodium dodecyl sulphate polyacrylamide gel electrophoresis of seed proteins. These were distinguished into ten groups on different banding patterns in four zones (A, B, C and D). The UPGMA analysis showed that VRP-32, VRP-16 and PMR-53 and PMR-19, Arka Ajit, PSM-4, PMR-62, PMR-31, PMR-60, Nepal Pea, VP-266, PMR-32, E-6, AP-3, Arkel, PSM-3 and VL-7 formed two different clusters. However, PSM-4, PMR-62, PMR-31 and PMR-60; Nepal Pea, VP-266, PMR-32 and AP-3; Arkel, PSM-3 and VL-7 were three different neighbouring groups. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/74141 | |
| dc.language.iso | en | en_US |
| dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand) | en_US |
| dc.sub | Vegetable Science | |
| dc.subject | genetic analysis, combining ability, heterosis, proteins, profiling, peas, horticulture, Pisum sativum, vegetables | en_US |
| dc.these.type | M.Sc | |
| dc.title | Genetic trends in combining ability, heterosis and protein profiling in vegetable pea (Pisum sativum L.) | en_US |
| dc.type | Thesis | en_US |