STANDARDIZATION OF GERMINATION TEST PROCEDURE IN MEDICINAL PLANT SPECIES
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Date
2001-08-04
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK BANGALORE
Abstract
Studies on four medicinal plant species viz., Ammi majus,
Phyllanthus emblica, Plantago ovata and Psorelia corylifolia was
undertaken to standardize media and temperature required to test for
germination in the laboratory. The experiment was comprised of nine
temperature ranges involving four constant(20", 25", 30°, 35° C) and
four alternate (20-30°, 25-35°, 20-35°, 15-25° C) temperatures along
with one ambient temperature. The three media were Between Paper
(BP), Top of Paper (TP) and Sand (S).
Among the different temperatures and media studied, the
maximum germination (95.50%) was observed in Ammi majus at 20-0
temperature in BP method. The first and final count were fixed on 6th
and 10th day, respectively. In sand medium at alternate temperature of
20-30°C Phyllanthus amblica recorded maximum germination (95.00%).
This was found on par with 25-35=0 temperature. The first and final
count can be fixed on 5th and 10th day, respectively. The alternate
temperature of 15-25=0 in BP method recorded highest germination
(96.00%) in Plantago ovata. The first and final count can be fixed on 2nd
and 5th day, respectively in BP method. In Psorelia corylifolia highest
germination (95.00%) was observed in sand medium at 15-25=0. The
first and final count can be fixed on 2nd and 5th day, respectively.
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