STUDIES ON THE PROTECTIVE AND ANTIOXIDANT EFFECTS OF AN APHRODISIAC HERB “TRIBULUS TERRESTRIS” ON CADMIUM INDUCED TESTICULAR DAMAGE.
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Date
2010-06
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI – 517 502. (A.P) INDIA
Abstract
ABSTRACT :
The present study was aimed to evaluate the protective effect of Tribulus terrestris (TT) on Cadmium (Cd) induced toxicity in rats. Forty adult male rats were divided into 4 groups with ten in each group and treatment given as follows: group I (Control) received double distilled water; group II (Cd toxic) received CdCl2 @ 3 mg/kg b.wt s/c at weekly interval for 6 weeks, group III (TT treatment) received CdCl2 @ 3 mg/kg b.wt s/c at weekly interval for 6 weeks and a daily oral dose of ethanolic extract of TT @ 5 mg/kg b. wt and group IV (Vit-E treatment) received CdCl2 @ 3 mg/kg b.wt s/c at weekly interval and daily oral dose of Vit-E @ 75 mg/kg b. wt. At the end of 6th week rats were sacrificed and testes were collected and estimated the organ weight, testicular tissue peroxidation markers, antioxidant markers, and functional markers, concentration of Cd, histomorphometry and histopathology.
Phytochemical screening of ethanolic extract of TT revealed the presence of flavonoids, tannins, saponins, polyphenols and steroids. In Cd toxic group rats weekly average body weight gain and testicular weights were significantly reduced. The testicular tissue antioxidants such as SOD, CAT and GSH were significantly reduced; the peroxidation markers such as TBARS and protein carbonyls were significantly increased and the functional markers such as ALP and LDH were also significantly reduced in Cd administered group. Cd significantly altered the histological structures; histomorphometry revealed significant reduction in number of spermatogonia, resting spermatocytes, pachytene spermatocytes, spermatids, and leydig cells in seminiferous tubules. Seminiferous tubular diameter was also reduced along with pathological changes of disruption of basement membrane and tubular epithelium, formation of gaint cells, vacuolation in Sertoli cells. Treatment of Cd toxic rats with ethanolic extract of TT significantly reversed the weekly body weight gain, testicular weights, testicular tissue peroxidation markers, antioxidant markers and functional markers and also significantly reversed the histopathological and histomorphometric observations. Vitamin-E treatment to Cd intoxicated rats all the antioxidant, peroxidation and functional markers reversed significantly compared to the Cd toxic group and also to the TT treated group. Histoarchitecture was near normal. With this study, it is concluded that Cd accumulates in testicular tissues and induced toxicity through induction of oxidative stress. Supplementation of TT was offered moderate protection compared to Vitamin E.
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