STUDIES ON IMPROVEMENT OF SYMBIOTIC NITROGEN FIXATION IN Albizia procera (Roxb.) Benth. UNDER MID HIMALAYAN REGION

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dc.contributor.advisorRANA, NEERJA
dc.contributor.authorMEENAKSHI
dc.date.accessioned2019-11-15T07:25:07Z
dc.date.available2019-11-15T07:25:07Z
dc.date.issued2019-11
dc.description.abstractABSTRACT The intensive harvesting of forest resources have led to decreased nitrogen and organic matter content in the forest soils. Biological nitrogen fixation (BNF) is a cheap renewable nitrogen source, essential to the sustainable productivity of afforested sites. Biological nitrogen fixation offers economically and ecologically sound means for reducing the requirement of nitrogenous fertilizer, thereby restoring the degraded ecosystem. The present investigation entitled “Studies on improvement of symbiotic nitrogen fixation in Albizia procera (Roxb.) Benth. under mid Himalayan region” was carried out in the Microbiology Laboratory of Department of Basic Sciences during the years 2016-2019. Results of the study revealed that among the 10 different seed sources, Baddi seed source of Himachal Pradesh and FRI seed source from Uttrakhand were found best for pod, seed germination and seedling growth parameters. It is evident from the studies that larger seeds have greater per cent germination than smaller seeds. Non significant variation between pod and seed traits with geographical factors were noticed which indicated non-clinal variation. In total of 66 isolates, 38 from Himachal Pradesh and 28 from Uttrakhand were isolated fromroot nodules of Albizia procera seedlings. On the basis of authentication tests only 38 isolates were confirmed as rhizobia. Among these 38 rhizobial isolates, 29 isolates were Psolubilizers, 27 isolates were siderophore producers and 19 isolates were HCN producers. Among the 21 rhizobial isolates from Himachal Pradesh maximum P-solubilization (263.23µg/ml), siderophore production (106.30%) and IAA (82.75µg/ml) was recorded with rhizobial isolate BA2. Whereas, maximum P-solubilization (261.00µg/ml), siderophore production (108.12%) and IAA (85.52µg/ml) was recorded with rhizobial isolate FA6 from Uttrakhand. Rhizobial isolates BA2 and FA6 also showed high per cent growth inhibition against 6 fungal pathogens. On the basis of plant growth promoting traits and antifungal activities, two isolates BA2 and FA6 were selected and applied as biofertilizers. Both the biofertilizers with 20kg/ha nitrogen significantly increased shoot biomass, root biomass, nodulation status and available N P K content of soil over uninoculated control. These isolates were identified as Rhizobium leguminosarum (BA2) and Rhizobium alamii (FA6). The application of charcoal based bio-inoculant of rhizobial strain FA6+20 kg/ha nitrogen has not only significantly influenced growth characters and nitrogen fixation potential of Albizia procera seedlings but also improved the available N P K content of the soil. Hence, application of charcoal based bioinoculant with rhizobial strain FA6 have enormous potential to be used as biofertilizer for enhanced growth, nodulation status and protection of A.procera seedlings and to sustain soil health.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810135378
dc.keywordsnitrogen and organic ,Biological nitrogen fixation,cheap renewable nitrogen source, harvesting of forest resourcesen_US
dc.language.isoenen_US
dc.pages149+xen_US
dc.publisherUHF,NAUNIen_US
dc.subBasic Sciencesen_US
dc.subjectnullen_US
dc.themenitrogen and organic ,Biological nitrogen fixation,cheap renewable nitrogen source, harvesting of forest resourcesen_US
dc.these.typePh.Den_US
dc.titleSTUDIES ON IMPROVEMENT OF SYMBIOTIC NITROGEN FIXATION IN Albizia procera (Roxb.) Benth. UNDER MID HIMALAYAN REGIONen_US
dc.typeThesisen_US
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