Toxic and anti-fertility effect of Calotropis procera leaf extract on Bandicota bengalensis
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Date
2019
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Punjab Agricultural University, Ludhiana
Abstract
Calotropis procera has strong insecticidal, fungicidal, toxic (hepatotoxic, nephrotoxic and
cardiotoxic) and anti-fertility effects But no data is available regarding its toxic and
antifertility effect on male Bandicota bangalensis. Three different C. procera dry leaf powder
extract and fresh leaf juice based treated baits were tested against male bandicoot rat, B.
bengalensis in bi-choice feeding tests. Results demonstrated no lethal or acute toxic effects
with all C. Procera leaf based treated baits when given to rats continuously for three days.
Therefore, the duration of experiment was increased to 30 days to determine the anti-fertility
and chronic toxicity effects of same treated baits as used in acute toxicity determination
experiment. There was a significant reduction in consumption (g/100 g b wt.) of treated baits
as compared to untreated bait. Out of three treated baits, average consumption of active
ingredient was maximum with treated bait 2 and percent acceptance of this bait ranged from
47.66 % to 60.67 %. Results also revealed significant reduction in the weights of male
reproductive organs (testes, cauda epididymis, corpus epididymis and caput epididymis),
sperm motility, viability and sperm concentration and increase in sperm abnormalities in rats
fed on treated bait 2. The histomorphological study of testis revealed significant reduction in
the diameter of seminiferous tubules and thickness of germinal epithelium because of
reduction in number of both germ and Sertoli cells in different stages of seminiferous
epithelial cycle in rats fed on treated bait 2 indicating efficacy of treated bait 2 as antifertility
agent. However nephrotoxic effect and oxidative stress was not recorded in same rats fed on
treated bait 2.
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