ISOLATION AND CHARACTERIZATION OF LISTERIA SPECIES FROM POULTRY MEAT
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Date
2009
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Anand Agricultural University, Anand
Abstract
The present study is undertaken with intension of isolating and identifying
the Listeria spp. with special reference to L. monocytogenes from raw poultry meat
sold at retail meat shops in Anand, Gujarat.
The samples of raw poultry meat consist different edible parts of chicken
such as breast, leg, liver, gizzard and wing (35 each) collected aseptically from local
meat market and subjected to two stage enrichment process with UVM 1 and UVM 2
for two days each, followed by plating on three selective media viz. DRIA,
PALCAM and Oxford media. The colonies showing typical colony characteristics
were further characterized on the basis of their morphological and biochemical
characteristics. Cultures identified as Listeria spp. were further subjected to in vitro
pathogenicity (hemolysis on sheep blood agar and CAMP test), detection of different
virulence associated genes and sensitivity of Listeria spp. to various antibiotics
commonly used in human and veterinary treatment
Description
Keywords
Veterinary Public Health, Study