CHARACTERIZATION OF cry GENES IN NATIVE Bacillus thuringiensis ISOLATES
Loading...
Files
Date
2000
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
University of Agricultural Sciences, Dharwad
Abstract
"The present sUicly was concluded lo isolalc Baciffus thuringiensis from soils o f Westeni Ghat region in Uttara Kannada district o f Karnataka, India, to assess their insecticidal activity, molecular diversity and to identify specific cry genes in them. Out o f the initial 41 isolates, only ten isolates had endospores and crystals. Three isolates i.e., D l , D3 and D 2 I were toxic to diamond back moth {l^lu(ella xylos lella) larvae. Among these isolates D l caused the highest mortality (9 0% ) followed by D3 (8 0% ) and D21 (7 0%) . The isolates varied in sensitivity to antibiotics. All the three isolates, reference strain Bt -4 2 and reference isolate P I were sensitive to tetracycline, chloramphenicol and kanamycin. D l , D3 and D21 were resistant to ampicillin (1 5 0 ppm) and spectinomycin. In addition, D3 was also resistant to nalidixic acid (5 0 ppm). SDS-PAGF'. protein profile indicated the presence o f 130 kD a and 65 kD a bands in D l , D3, Bt -42 and P I . In D21, a 26 kD a protein was present. The genetic distance (% ) based on protein profile indicated the minimum genetic distance o f 16 .6 7% between PI and D l and the maximum genetic distance o f 8 0 .0 0% uciwccn D i and D2 1 . All the isolates had only one plasmid o f about 15 kb. The presence o f c ry genes in the isolates was detected based on the nature o f the PGR amplification products. Wi th c r y specific primers it was found that gene c ry !('. was present in all the three isolates D l , D3 and D21. c ry IA(c) gene was present in PI and D I and the nematode activc c ry gene was detected in P i , D l and D3."
Description
Keywords
null
Citation
No. of Reference 56