Genetic variability in BC1F2 and F3 wheat genotypes derived from WH 1105 x Kharchia 65 for salinity tolerance
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Date
2017
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CCSHAU
Abstract
Soil salinity is one of the major abiotic stress having multifarious adverse effects on all growth stages. In present study, WH1105 x Kharchia 65 derived BC1F2 and F3 and BC2F1 generations were evaluated for various morpho-physiological traits under salt stress and field conditions. Marker assisted selection confirmed the presence of Nax1 and Nax2 markers in 98 and 49 plants respectively for salinity tolerance. Twenty nine plants comprising of 16 BC1F2, 6 F3 and 7 BC2F1 were found to have both Nax1 and Nax2 loci. On the basis of phenotypic and genotypic variations fifty-four high yielding plants were selected for genotyping using polymorphic SSR markers. Out of total 149 SSR markers screened, 24 polymorphic SSR markers were used to produce molecular diversity among 54 selected plants. Cluster tree analysis of parents and BC1F2, F3, and BC2F1 plants showed that all the selected plants were inclined toward recurrent parent WH1105 representing higher similarity with WH1105. Six best plants were selected as high grain yielding and salt tolerant plants i.e. plant number 53C from BC1F2, 140B from F3 and 13af, 13bf, 14bf, 15af from BC2F1 and these plants could be further backcrossed with the parent WH1105 to develop salt tolerant wheat lines.
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