Exploring acute pancreatitis vis-à-vis experimental L-arginine induced acute pancreatitis and its amelioration by Phyllanthus emblica and Morus alba
| dc.contributor.advisor | Mahesh Kumar | |
| dc.contributor.author | Radhika | |
| dc.date.accessioned | 2019-12-31T09:35:47Z | |
| dc.date.available | 2019-12-31T09:35:47Z | |
| dc.date.issued | 2019-09 | |
| dc.description.abstract | The present study was conducted to evaluate ameliorating effect of fruits of Phyllanthus emblica and leaves of Morus alba on L-arginine induced acute pancreatitis in rats. Hydromethanolic extracts of M. alba leaves and P. emblica fruits were prepared and quantitative phytochemical analysis of these extracts was done. Total phenolic, total flavonoid and total flavonol content in P. emblica extract were110.92 ± 2.55 mg GAE/g, 23.36 ± 1.11 mg QE/g and 20.61 ± 1.06 mg QE/g, respectively. However,total phenolic, total flavonoid and total flavonol contents in M. alba extract were 31.26 ± 1.68 mg GAE/g, 11.45 ± 0.76 mg QE/g and 9.45 ± 1.25 mg QE/g, respectively. High performance liquid chromatography revealed presence of gallic acid (18 μg/g), quercetin (260 μg/g), rutin (35.8 μg/g) and trans-ferulic acid (14.8 μg/g) in P. emblica extract and gallic acid (12.4 μg/g), rutin (10 μg/g) and trans-ferulic acid (6.8 μg/g) in M. alba extract. In vitro evaluation of antioxidant activity of plant extracts was done using DPPH assay and total antioxidant assay. In vitro evaluation of anti-inflammatory activity of plant extracts was done by inhibition of protein denaturation and proteinase inhibitory action assays. IC50 values of extracts of P. emblica fruits and M. alba leaves were 221.21 and 361.38 μg/ml, respectively on basis of inhibition of protein denaturation assay. Whereas, IC50 values of extracts of P. emblica fruits and M. alba leaves were 292.9 and 382.77 μg/ml, respectively on basis of proteinase inhibitory action. Acute pancreatitis was induced in rats by administration of L-arginine @ 2 g/kg i.p. at 1 h interval. Prepared extracts of P. emblica and M. alba were given @ 200 mg/kg and 400 mg/kg, respectively p.o. daily to rats in two separate groups. Haematolgy, serum biomarkers of pancreas, kidney and heart, oxidative stress in erythrocytes and pancreas, lungs, kidney and heart, histopatholgical examination were used to access therapeutic efficacy. L-arginine administrationproduced acute necrotizing pancreatitis in rats which was associated with production of inflammatory mediators (IL-6 and TNFα) and production of marked oxidative stress not only in pancreas, but also in other organs viz. lungs, kidney and heart. Histopathologic examination showed that L-arginine administration produced acute necrotizing pancreatitis in rats with early signs of congestion, haemorrhage and vacuolationupto6hr followed by necrosis prominent at 24 hours and reaching its peak at 3 days interval. Apart from pancreatitis, pronounced effects on heart (myocardititis, hydropic degeneration, cardiac fiber degeneration), kidneys (haemorrhage, congestion, inflammation and tubular degeneration) and lungs (congestion, haemorrhage and thickening of interstitial septum, increased cellular counts in BALF) were also noticed. Treatment with P. emblica and M. alba extracts elucidated a protective effect by abolition of edema and fibrosis, as well by bringing haematological, biochemical and antioxidant indices to almost normal levels. These results were almost comparable with melatonin given as standard preventive therapy in acute pancreatitis. Thus, it can be concluded from results of the present study that P. emblica and M. alba extracts are effective in ameliorating acute necrotizing pancreatitis induced by L-arginine in rats. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810139138 | |
| dc.keywords | acute diseases, pancreatitis, arginine, amelioration, Phyllanthus emblica, gooseberries, medicinal plants,Morus alba | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 152 | en_US |
| dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand) | en_US |
| dc.research.problem | Acute Diseases | en_US |
| dc.sub | Veterinary Medicine | en_US |
| dc.subject | null | en_US |
| dc.theme | Medicinal Plants | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | Exploring acute pancreatitis vis-à-vis experimental L-arginine induced acute pancreatitis and its amelioration by Phyllanthus emblica and Morus alba | en_US |
| dc.type | Thesis | en_US |