STUDIES ON CERTAIN ASPECTS OF BOLIARY AMPHISTOMOSIS IN BUFFALOES (Babalus bubalis)
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Date
2006-02
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT:
We research work on "Studies on certain aspects of biliary
amphistornosis in buffaloes" was under taken to study the prevalence of
gigantocotylosis in naturally infected buffaloes, based on dung sample
and slaughter house examination. Age, sex, breed, season wise
prevalence at certain places of Chittoor and Nellore districts of Andhra
Pradesh were surveyed-$ total of 221 buffaloes were examined in the
present study. The present of prevalence in buffaloes was 38.91 per cent.
Among the 12 places choosen the prevalence was more in Tirupati
(41.67%) least in Chittoor (12.5%) in Chittoor District, whereas in
Nellore District more prevalence was recorded in Nellore (48.15%) and
least in Podalakur (35.71 %).The prevalence of biliary amphistomosis was more in above 4
years agc group (45%) and in females (40%). The prevalence was more
in non-descriptive animals (40.68%) and also in rainy season (46.15%).
Attempts were made to observe certain biochemical changes in -
serum of naturally infected buffaloes. Serum total proteins (6.792 g/100 ml),
albumins (3.256 g/ dl) and A/G ratio (0.921 g/dl) were decreased where
as globulins (3.536 g/dl), conjugated bilirubin (0.093 g/dl) and total
bilirubin (0.805 ddl) wcre increased.Attempts wcre madc to diagnose gigantocotylosis in naturally infected buffaloes by carrying out three sero diagnostic tests viz., counter
current immunoelectrophoresis, ELISA and Dot - ELISA. The whole
worm extract of antigen was prepared and the protein content of this
antigen was prepared and the protein content of this antigen was
estimated to be 11.2 mg/ml. Hyperimmune serum was raised against
whole worm extract of Gigantocotyle explanaturn antigen, and serum
samples selected randomly from naturally infected buffaloes with
Gigantocotyle explanaturn were tested against whole worm extract of
Gigantocotyle explanaturn. The CIEP, Dot - ELISA and ELISA tests
showed a sensitivity of 85.8, 92 and 98 per cent respectively in detecting
biliary amphistornosis in naturally infected buffaloes.
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