ELUCIDATING THE EFFICACY OF SODIUM ALGINATE AS AN ANTIBACTERIAL ALTERNATIVE IN CRYOPRESERVATION OF SAHIWAL BULL SEMEN
dc.contributor.advisor | NISHANT KUMAR | |
dc.contributor.author | MOHIT KUMAR | |
dc.date.accessioned | 2023-12-04T09:57:04Z | |
dc.date.available | 2023-12-04T09:57:04Z | |
dc.date.issued | 2022 | |
dc.description.abstract | The present study was conducted to investigate the efficacy of sodium alginate (S.A.) as an antibacterial alternative in cryopreservation of Sahiwal bull semen. In 1st objective, each of the pre-dominant Gram –ve (Proteus spp.) & Gram +ve (Bacillus spp.) bacterial isolates from Sahiwal bulls were collected and the antibacterial potency of sodium alginate was determined. Minimum inhibitory concentration (MIC) was determined by the broth microdilution method, in which two-fold serial dilutions of S.A. were done from 10 to 0.078 mg/ml concentration. It was found that the MIC of S.A. against Proteus spp. & Bacillus spp. was 0.625 and 1.25 mg/ml respectively. Similarly, the minimum bactericidal concentration (MBC) of S.A. against Proteus spp. & Bacillus spp. was found to be 0.625 and 2.5 mg/ml respectively. In 2nd objective, effect of S.A. as an antibacterial alternative on microbial load and freezability of Sahiwal bull semen was studied. Dose tolerance test in semen, as a pilot study was done by using six different S.A. concentrations (without streptopenicillin) based on MIC doses keeping one group as negative control (without streptopenicillin). Among these treated groups, three best doses (i.e. 1.25, 0.625 & 0.313 mg/ml) were selected on the basis of progressive motility & CFU/ml count for the standardization experiment. In standardization experiment, extended ejaculates were split into 3 treatment groups (1.25 mg/ml T1; 0.625 mg/ml T2; and 0.353 mg/ml T3- no antibiotics) and one control (Penicillin-1000IU/ml; Streptomycin-1mg/ml) group. Post-dilution, pre-freeze, post-thaw seminal parameters & bacterial load (CFU/ml) were evaluated, and T2 (0.625 mg/ml S.A.) was selected as the best dose for the final experiment. In the final experiment, ejaculates were split into 3 groups; one was kept as control (Penicillin-1000IU/ml; Streptomycin-1mg/ml), T1 (0.625 mg/ml S.A., no antibiotics) and T2 (0.625 mg/ml S.A. + antibiotics) groups. It was found that progressive motility, viability, HOST and acrosomal integrity of spermatozoa in the post-thaw stages was found to be significantly higher (p<0.05) in the T2 group as compared to the control & T1 group. Bacterial load (CFU/ml) at all the cryopreservation stages was found to be significantly lower (p<0.05) in the T2 group as compared to the control. Cryo-capacitation were significantly (p<0.05) reduced in the T2 group as compared to the control and T1 group at the post-thaw stages. No significant difference (p<0.05) was recorded in the protamine deficiency of the spermatozoa between the control, T1 & T2 groups at post-thaw stages. In the CASA study, total motility (TM), and progressive motility (PM) was found to be significantly higher (p<0.05) in T2 as compared to the control and T1 group. While there was no significant difference recorded in curvilinear velocity (VCL), straight linear velocity (VSL), average path velocity (VAP), and beat cross frequency (BCF) in the control and T2 groups. It can be concluded that S.A. has limited anti-bacterial activity as compared to strepto-penicillin, however it potentiates the antibacterial activity of strepto-penicillin if used in the supplementation. Also, S.A. in supplementation with antibiotics, enhances progressive motility, viability, plasma membrane integrity and acrosomal integrity of the sperm, as it protects the sperm from the adverse effects of cryopreservation. | |
dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810202087 | |
dc.language.iso | English | |
dc.pages | 73 p. | |
dc.publisher | ICAR-NDRI, KARNAL | |
dc.sub | Veterinary Gynaecology and Obstetrics | |
dc.theme | Veterinary Gynaecology and Obstetrics | |
dc.these.type | M.V.Sc. | |
dc.title | ELUCIDATING THE EFFICACY OF SODIUM ALGINATE AS AN ANTIBACTERIAL ALTERNATIVE IN CRYOPRESERVATION OF SAHIWAL BULL SEMEN | |
dc.type | Thesis |