EFFECT OF OSMOTIC STRESS ON OSMOLYTE ACCUMULATION AND AMMONIA ASSIMILATING ENZYMES IN CHICKPEA CULTIVARS
| dc.contributor.advisor | LOKHANDE, P. K. | |
| dc.contributor.author | Pawar, Vaishali Vinayak | |
| dc.date.accessioned | 2017-11-13T09:00:46Z | |
| dc.date.available | 2017-11-13T09:00:46Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | The present research work entitled “Effect of osmotic stress on osmolyte accumulation and ammonia assimilating enzymes in chickpea cultivars” was carried out with a view to evaluate the effect of PEG-6000 induced osmotic stress on nine chickpea cultivars. The four drought tolerant viz., ICC-4958, Vijay, Digvijay, PG-70-52 and five drought susceptible cultivars viz., PG09103, ICC-11117, ICCGP-11112, JG-216 and PG-1808 were subjected to mild (-0.4MPa) and severe (-1.2MPa) osmotic stress induced by PEG-6000. The level of relative leaf water content (RLWC), chlorophyll stability index (CSI), membrane stability index (MSI), ammonia assimilation enzymes viz. nitrate reductase (NR), glutamine synthetase (GS), glutamate synthase (GOGAT), proline, ∆1 pyrroline-5-carboxlate synthetase (P5CS) activity, glycine betaine and mannitol content were estimated. The RLWC, CSI, MSI significantly decreased in both drought tolerant and drought susceptible cultivars of chickpea under mild and severe osmotic stress conditions. The levels of osmolytes proline, P5CS, glycine betaine and mannitol were significantly increased in both drought tolerant and susceptible chickpea cultivars under mild and severe osmotic stress condition. The extent of increase was higher in tolerant than susceptible cultivars. The activity of P5CS, a rate limiting enzyme in proline biosynthesis also increased under mild and severe stress. The activity of ammonia assimilating enzymes viz., NR, GS, GOGAT was significantly decreased in both tolerant and susceptible cultivars of chickpea under mild and severe osmotic stress condition as compared to control condition and the rate of reduction was higher in susceptible than tolerant cultivars. ICC-4958 recorded higher accumulation of osmolytes viz., proline, glycine betaine, mannitol and P5CS activity with less decrease in RLWC, CSI, MSI, NR, GS, GOGAT. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810035504 | |
| dc.keywords | CHICKPEA | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 106; PP | en_US |
| dc.publisher | MPKV, UNIVERSITY LIBRARY RAHURI | en_US |
| dc.sub | Agricultural Biochemistry | en_US |
| dc.subject | null | en_US |
| dc.theme | - | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | EFFECT OF OSMOTIC STRESS ON OSMOLYTE ACCUMULATION AND AMMONIA ASSIMILATING ENZYMES IN CHICKPEA CULTIVARS | en_US |
| dc.type | Thesis | en_US |