RELATIVE EFFICIENCY OF IMPERATA CYLINDRICA- AND MAIZEMEDIATED CHROMOSOME ELIMINATION SYSTEMS FOR HAPLOID INDUCTION IN VARIOUS DERIVATIVES OF NEWLY SYNTHESIZED INDIGENOUS TRITICALES AND BREAD WHEAT GENOTYPES
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Date
2021-11-30
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Palampur
Abstract
The research endeavour entitled “Relative efficiency of Imperata cylindrica- and maizemediated chromosome elimination systems for haploid induction in various derivatives of newly
synthesized indigenous triticales and bread wheat genotypes” was executed in the Department of
Genetics and Plant Breeding, CSK HPKV, Palampur during the years 2019-20 and 2020-21 with the
goal to develop triticale × wheat F1 & BC1F1 to compare hybridization potential of diverse
combinations and work out relative efficiency of haploid induction in triticale × wheat derivatives
following Imperata cylindrica and maize- mediated chromosome elimination technique of doubled
haploidy breeding. The material for present investigation comprised of 10 bread wheat genotypes HPW
155, HPW 249, HPW 349, HPW 368, HPW 440, VWFW 858, VWFW 896, Tyari 1, Saptdhara and DH
114; two newly synthesized triticale genotypes, Triticale 1 and Triticale 2 and their F1s & backcross
derivatives. During rabi 2019-20, hybridization work was carried out using wheat genotypes as female
parent and triticale genotypes as male parent followed by estimating the frequency of seed setting
induced by the triticale genotypes in wheat parental lines. The first filial generation produced after
hybridization was sown at HAREC, Kukumseri to deduce the frequency of seed setting for the
formation of backcross generation. Triticale 1 performed better than Triticale 2 for development of F1s
while Tyari 1 exhibited significantly higher response for hybridization with Triticale 1 and Triticale 2.
The mean seed setting frequency recorded for the development of F1 generation was 22.73% & 15.84%
with Triticale 1 and Triticale 2 respectively; while in BC1F1, it was 21.02% & 20.92% with Triticale 1
and Triticale 2 respectively. All the haploid induction parameters viz., pseudo seed formation, embryo
formation and haploid regeneration, showed varied response in each generation of triticale x wheat
derivatives exhibiting genotype specificity for haploid induction. On the basis of statistical analysis, it
was revealed that relative efficiency of I. cylindrica was higher than maize for haploid induction in
triticale x wheat derivatives. Derivatives of bread wheat genotypes with both the triticale genotypes
preformed at par for haploid induction following I. cylindrica and Z. mays mediated- chromosome
elimination technique. Backcross generation performed better than F1 and F2 genotypes towards
haploid induction parameters. This attempt of hybridizing wheat with triticale; producing haploids from
their F1, F2 and BC1F1, which can be transformed into double haploids following colchicine treatment
has paved way to accelerate the wheat improvement programme with enhanced precision using the
desirable genes from rye into wheat using triticale as bridging species.