Isolation, identification and evaluation of indigenous fungal bio-agents against Meloidogyne incognita in okra
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Date
2021-07
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CCSHAU, Hisar
Abstract
Investigations were carried out for the Isolation, identification and evaluation of indigenous
fungal bio-agents against Meloidogyne incognita in okra both under in-vitro and pot house conditions.
Under in-vitro condition, the effect of fungal isolates on egg hatching inhibition and juvenile mortality
of Meloidogyne incognita was assessed at various concentrations, i.e., 20%, 40%, 60% and 80%.
Among all the isolates, Trichoderma asperellum FbMi-6 gave significantly highest and maximum
mortality of root-knot nematode juvenile followed by Trichoderma asperellum FbMi-4, at all the four
concentrations. The highest mortality of juveniles was observed at 80% concentration. For hatching
inhibition of root-knot nematode at all the different concentrations, i.e., 20%, 40%, 60% and 80%
showed that all fungal isolates inhibited the hatching of root-knot nematode as compared to the
untreated check. Significantly highest and maximum egg hatching inhibition was observed in
Trichoderma asperellum FbMi-6 followed by Trichoderma asperellum FbMi-4. The rate of juvenile
hatching of all the isolates was inversely proportional to the concentration of culture filtrate. The
nematicidal activity of the culture filtrates of Trichoderma isolates increased with the increase in the
concentration of the filtrates and the time of exposure. Under pot conditions, significantly highest plant
growth parameters (plant height, fresh shoot and root weight, dry shoot and root weight, germination
percentage and germination vigour) were achieved in neem cake enriched with T. asperellum FbMi-6
@ 20g kg-1 soil followed by neem cake enriched with T. asperellum FbMi-4 @ 20g kg-1 soil. Numbers
of galls per plant, number of eggs per egg mass, number of egg masses per plant, final nematode
population and reproduction factor were significantly lowest in T. asperellum FbMi-6 @ 20g kg-1 soil
followed by neem cake enriched with T. asperellum FbMi-4 @ 20g kg-1 soil. Higher amount of
polyphenols, total chlorophyll, nitrogen balance index, anthocyanin and flavonoids in neem cake
enriched with T. asperellum FbMi-6 @ 20g kg-1 soil as compared with untreated inoculated check.