Micropropagation of guggal (Commiphora mukul, hook ex stock)
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Date
2005
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Publisher
CCSHAU
Abstract
The present study was undertaken to develop an efficient
micropropgation system for plant regeneration and transfer the regenerated
plants to soil conditions in Guggal (Commiphora mukul, Hook ex stock).
Nodal, shoot tip and leaf explants were used for shoot regeneration on 37
different media supplemented with different growth regulators and additives.
Nodal explants gave best shoot regeneration response (71.40%) on MS medium
supplemented with kinetin (1.0 mg/l) and GA3 (1.0 mg/l) and MS medium
fortified with BAP (5.0 mg/l) and GA3 (1.0 mg/l) gave highest per cent of shoot
initiation (81.11%) in shoot tip explants. The shoots were elongated on MS
medium containing different concentrations of growth regulators using agar as
gelling agent. Best shoot elongation was observed on MS medium containing
BAP (5.0 mg/l) and GA3 (1.0 mg/l) out of the four media tried. Shoot tip
explants are considered to be the best explant compared to nodal explants for
shoot regeneration. Leaf explants did not show any response. However,
callusing was observed on MS medium containing 2,4-D (1.0 mg/l). Analysis
of variance of shoot formation indicated that nature of explants, medium and
their interaction were highly significant at 1 per cent level of significance.
Rooting of regenerated shoots was observed (35.00%) on liquid MS medium
supplemented with IBA (1.0 mg/l) out of fifteen different media tried. The
regenerated plantlets were transferred to soil and vermculite mixture (1:1) and
30 per cent plants survived in the soil.