STORAGE QUALITY OF LIQUID SEMEN OF LARGE WHITE YORKSHIRE BOAR SUPPLEMENTED WITH CHOLESTEROL LOADED CYCLODEXTRIN
| dc.contributor.advisor | Harshan, Hiron M. | |
| dc.contributor.author | T., SHYLESH | |
| dc.date.accessioned | 2021-01-12T04:57:37Z | |
| dc.date.available | 2021-01-12T04:57:37Z | |
| dc.date.issued | 2019-09-04 | |
| dc.description.abstract | The present study was carried out to assess the effect of supplementation of cholesterol loaded cyclodextrin on quality of liquid semen of Large White Yorkshire (LWY) boar stored at 15C. A total of 12 ejaculates were used for the study from four adult healthy LWY boars maintained at Centre for pig production and research, Mannuthy. The fresh semen characteristics were evaluated immediately after collection and semen was extended with Beltsville thaw solution to a final concentration of 120 × 106 sperms/mL. The extended semen was split into five groups and processed as Group I (without any supplementation); Group II (supplemented with cholesterol loaded cyclodextrin (CLC) at the rate of 0.75 mg/120 million sperms); Group III (supplemented with CLC at the rate of 1.5 mg/120 million sperms); Group IV (supplemented with CLC at the rate of 3 mg/120 million sperms) and Group V (supplemented with methyl- cyclodextrin at the rate of 1.5 mg/120 million sperms). The processed semen was gradually cooled to 22°C and held at same temperature for three hours. The semen was subsequently cooled to 15°C and stored for 72 h and evaluated at 4, 24, 48 and 72 h of storage for semen attributes. The progressive motility of semen was significantly (p < 0.01) higher in group III and IV at 4h of storage and in group III at 24h, 48 h and 72 h of storage. The sperm viability was significantly higher (p < 0.01) in group III at 4, 24, 48 and 72 h of storage. The sperm abnormality was significantly (p < 0.01) higher in MBCD treated group at 48 and 72 h of storage. The HOS response was significantly (p < 0.01) higher in group II and III at all stages of preservation. The acrosome integrity was significantly (p < 0.01) higher in group III at 24 and 48 h, whereas both group II and III had significantly (p < 0.01) higher values at 72 h of preservation. The cholesterol content was significantly (p < 0.01) higher in group IV at 72h. The study revealed that cholesterol treatment of the spermatozoa with means of CLC at a rate of 1.5 mg/120 million sperms improved the quality of stored spermatozoa at various stages of storage at 15C till 72 h. It was also found that the supplementation of cholesterol as CLC could increase the amount of sperm cholesterol. Thus the positive effects of CLC on sperm characteristics during storage at 15C could be due to the supplementation of cholesterol to sperm membrane. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810159828 | |
| dc.keywords | WHITE YORKSHIRE BOAR, CYCLODEXTRIN, LIQUID SEMEN , CHOLESTEROL LOADED CYCLODEXTRIN (CLC), SPERMATOZOA SPERM CHOLESTEROL, | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 94 | en_US |
| dc.publisher | COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD | en_US |
| dc.research.problem | STORAGE QUALITY OF LIQUID SEMEN OF LARGE WHITE YORKSHIRE BOAR SUPPLEMENTED WITH CHOLESTEROL LOADED CYCLODEXTRIN | en_US |
| dc.sub | Animal Reproduction, Gynaecology and Obstetrics | en_US |
| dc.theme | STORAGE QUALITY OF LIQUID SEMEN OF LARGE WHITE YORKSHIRE BOAR SUPPLEMENTED WITH CHOLESTEROL LOADED CYCLODEXTRIN | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | STORAGE QUALITY OF LIQUID SEMEN OF LARGE WHITE YORKSHIRE BOAR SUPPLEMENTED WITH CHOLESTEROL LOADED CYCLODEXTRIN | en_US |
| dc.type | Thesis | en_US |