STORAGE QUALITY OF LIQUID SEMEN OF LARGE WHITE YORKSHIRE BOAR SUPPLEMENTED WITH CHOLESTEROL LOADED CYCLODEXTRIN
Loading...
Date
2019-09-04
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD
Abstract
The present study was carried out to assess the effect of supplementation of
cholesterol loaded cyclodextrin on quality of liquid semen of Large White Yorkshire
(LWY) boar stored at 15C. A total of 12 ejaculates were used for the study from
four adult healthy LWY boars maintained at Centre for pig production and research,
Mannuthy. The fresh semen characteristics were evaluated immediately after
collection and semen was extended with Beltsville thaw solution to a final
concentration of 120 × 106
sperms/mL. The extended semen was split into five
groups and processed as Group I (without any supplementation); Group II
(supplemented with cholesterol loaded cyclodextrin (CLC) at the rate of 0.75
mg/120 million sperms); Group III (supplemented with CLC at the rate of 1.5
mg/120 million sperms); Group IV (supplemented with CLC at the rate of 3 mg/120
million sperms) and Group V (supplemented with methyl- cyclodextrin at the rate
of 1.5 mg/120 million sperms). The processed semen was gradually cooled to 22°C
and held at same temperature for three hours. The semen was subsequently cooled to
15°C and stored for 72 h and evaluated at 4, 24, 48 and 72 h of storage for semen
attributes. The progressive motility of semen was significantly (p < 0.01) higher in
group III and IV at 4h of storage and in group III at 24h, 48 h and 72 h of storage.
The sperm viability was significantly higher (p < 0.01) in group III at 4, 24, 48 and
72 h of storage. The sperm abnormality was significantly (p < 0.01) higher in
MBCD treated group at 48 and 72 h of storage. The HOS response was significantly
(p < 0.01) higher in group II and III at all stages of preservation. The acrosome
integrity was significantly (p < 0.01) higher in group III at 24 and 48 h, whereas
both group II and III had significantly (p < 0.01) higher values at 72 h of
preservation. The cholesterol content was significantly (p < 0.01) higher in group IV
at 72h. The study revealed that cholesterol treatment of the spermatozoa with means
of CLC at a rate of 1.5 mg/120 million sperms improved the quality of stored
spermatozoa at various stages of storage at 15C till 72 h. It was also found that the
supplementation of cholesterol as CLC could increase the amount of sperm
cholesterol. Thus the positive effects of CLC on sperm characteristics during storage
at 15C could be due to the supplementation of cholesterol to sperm membrane.