MANAGEMENT OF OXIDATIVE STRESS IN CAPRINE ANAPLASMOSIS
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Date
2019-08-05
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COLLEGE OF VETRINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR
Abstract
Caprine anaplasmosis is an economically important tick-borne rickettsial
disease that affects goats all over the world. The present study entitled “Management
of oxidative stress in caprine anaplasmosis” apprehends the correlation between
parasitemia caused by Anaplasma spp. and oxidative stress indices and also the
management of oxidative stress in goats affected with anaplasmosis. Microscopic
examination of stained blood smears from 162 animals revealed inclusion bodies of
Anaplasma spp. in 24 cases. Genus specific PCR for Anaplasma spp. yielded positive
results in 22 cases and 10 in-contact animals. Major clinical signs noted were anorexia, pyrexia, dullness, pallor of mucous
membrane, drop in milk yield , diarrhoea and wasting. Haematological analysis
revealed anaemia with low TEC, Hb and VPRC. All the diseased animals showed a
significant fall in the mean values of antioxidants such as reduced glutathione,
superoxide dismutase and catalase and a significant increase in the level of lipid
peroxidation. It was found that the parasitemia and malondialdehyde levels were in
positive correlation whereas all the antioxidants were in negative correlation with
parsitemia. Out of the 22 animals positive for anaplasmosis both in blood smear and
PCR, 16 female non pregnant goats selected for study were divided into two groups
consisting of eight animals each. Animals belonging to group I and II were treated
with oxytetracycline dihydrate. In addition, animals of group II were supplemented
with vitamin E- selenium combination. Haematological and oxidative stress
parameters were rechecked on 10th day of treatment. Clinical response and
improvement in haematological parameters were equally evident in both the groups.
At the end of the study period, a significant reduction in malondialdehyde level and a
significant increase in mean value of superoxide dismutase were detected in group II.
While there was a significant reduction in lipid peroxidation and a significant
increase in superoxide dismutase and catalase values within both the groups after
treatment, reduced glutathione showed no significant difference within the group.
Description
Submitted in partial fulfillment of requirement for the degree of Master of Veterinary Science in Veterinary Clinical Medicine, Ethics and Jurisprudence