Evaluation of immunomodulatory and hepatoprotective potential of Chenopodium album Linn. in rats
| dc.contributor.advisor | Ahmad, A.H. | |
| dc.contributor.author | Verma, Manish Kumar | |
| dc.date.accessioned | 2019-01-11T09:06:35Z | |
| dc.date.available | 2019-01-11T09:06:35Z | |
| dc.date.issued | 2018-06 | |
| dc.description.abstract | The present study was carried out to investigate the ameliorating potential of Chenopodium album Linn. in combating cyclophosphamide induced immunosuppression. The phytochemical analysis of plant extract revealed the presence of alkaloids, flavonoids, saponins, phenolic compounds, reducing sugars, glycosides and proteins. The experimental designs compromised of eight groups with each group having six rats. Group I served as control, group II rats were administered cyclophosphamide @100 mg/kg b. wt. in group III levamisole was administered @ 50 mg/kg b wt. in group IV both cyclophosphamide and levamisole, group V and VI were administered hydroethanolic extract of Chenopodium album (CAHE) @ 200 and 400 mg/kg b. wt. respectively, and in group VII and VIII CAHE @ 200 and 400 mg/kg b. wt. was given along with cyclophosphamide @100mg/kg b wt. on 9th and 16th day of study respectively, for 28 days. The reduction in body weight and organ weight significantly decreased in cyclophosphamide group. However, reduction in body weight was restored in group VII and VIII. Cyclophosphamide exposure caused significant (P<0.05) reduction in Hb, PCV, MCV, MCH, MCHC, TLC and DLC as compared to other groups. A significant (P<0.05) decline in total protein, albumin, globulin and A: G was observed in group II as compared to control. CAHE treated groups V, VI, VII and VIII showed significant (P<0.05) amelioration in the level total proteins as compared to group II and at par with normal values in control showing ameliorative effect of CAHE. A significant (P<0.05) increase in triglycerides, cholesterol, creatinine, BUN, AST, ALT and ALP was observed in cyclophosphamide treated group II, which were restored by CAHE towards normal indicating amelioration of these parameters by CAHE. A significant (P<0.05) decrease in HA titre, total Ig level, DTH reaction, phagocytic index, neutrophil adhesion and immunoglobulin (IgM and IgG) was observed in cyclophosphamide treated group II, whereas, treatment with CAHE restored these parameters towards normal values in a dose dependent manner indicating immune stimulation by CAHE. Histopathological changes in liver were characterized by severe congestion of blood vessels, Kupffer cell proliferation, severe sinusoidal congestion leading to loss of sinusoidal spaces, accumulation of mononuclear cells around many congested blood vessels, severe degeneration and swelling of hepatocytes throughout the parenchyma in liver; severe depletion of lymphoid cells, reticular cell hyperplasia and depletion of red pulp. In kidney severe congestion of large blood vessels and interstitial hemorrhages, vacuolation of glomeruli of the renal tubular epithelial cells and infiltration of mononuclear cells in interstitium was observed. In brain, presence of degeneration of neurons brain; congestion of the large blood vessels and in lungs thickening of interalveolar septa due to mononuclear cells infilteration were observed in cyclophosphamide treated rats which were ameliorated by treatment with CAHE in a dose dependent manner after 28 days in rats. Thus, it can be concluded from the present study that hydroethanolic extracts of C. Album has hepatoprotective and immunostimulant potential against cyclophosphamide induced toxicity. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810090062 | |
| dc.keywords | Evaluation of immunomodulatory and hepatoprotective potential of Chenopodium album Linn. in rats | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 167 | en_US |
| dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand) | en_US |
| dc.research.problem | Rats | en_US |
| dc.sub | Veterinary Pharmacology and Toxicology | en_US |
| dc.subject | null | en_US |
| dc.theme | Medicinal Plants | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | Evaluation of immunomodulatory and hepatoprotective potential of Chenopodium album Linn. in rats | en_US |
| dc.type | Thesis | en_US |