MOLECULAR DIVERSITY OF RHIZOBIA ASSOCIATED WITH LEGUMINOUS TREE SPECIES GROWN UNDER DIFFERENT AGRO-CLIMATIC ZONES OF HIMACHAL PRADESH

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dc.contributor.advisorANJALI CHAUHAN
dc.contributor.authorGAURAV KUMAR
dc.date.accessioned2023-10-21T14:33:45Z
dc.date.available2023-10-21T14:33:45Z
dc.date.issued2023-05-31
dc.description.abstractABSTRACT The present investigations were conducted to determine the genetic diversity of rhizobia with plant growth promoting traits associated with A. lebbeck and L. leucocephala grown under different agro climatic zones of Himachal Pradesh by amplified ribosomal DNA restriction analysis (ARDRA). The preliminary identification based on different authentication tests, viz. congo red test, bromothymol blue test, growth in Hofer’s alkaline broth and ketolactose medium a total of 105 isolates were tentatively identified as Rhizobium sp. Out of 105 isolates, forty-four were P-solubilzers, forty-three were siderophore producers and sixty were HCN producers. Twelve showed antagonism against Rhizoctonia solani, thirteen against Fusarium oxysporum andfifteen against Pythium sp. The maximum P-solubilization (184.63 μg/ml) was recorded for isolate BiA3 and maximum IAA production (84.56 μg/ml) was noted for isolate MaA1. Maximum siderophore production with zone size of 12 mm was recorded with the isolate MaA1 on CAS medium in A. lebbeck. In L. leucocephala, the maximum P-solubilization (180.15 μg/ml) was recorded for isolate SoL9 and maximum IAA production (86.28 μg/ml) was noted for isolate SiL7. Genetic diversity of 33 most potential isolates of A. lebbeck and L. leucocephalawas studied using restriction enzymes AluI and MspI by ARDRA. 33 isolates were grouped into six and seven major phylotypes, respectively. Among 33 sequenced isolates, 15 isolates were Rhizobium, 2 isolates were Agrobacterium and 16 isolates were from another genus (Bacillus, Arthrobacter, Paenibacillus, Serratia and Glutamicibacter). Inoculation with PGP rhizobia in six different treatment combination showed that the application of R. leguminosarum BiA3 and R. jaguaris HrL2 had significant increase in shoot height, root length, root and shoot biomass, number of nodules, leghemoglobin content and nitrogenase activity over uninoculated control
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810199628
dc.keywordsMolecular diversity
dc.keywordsrhizobia
dc.keywordsplant growth
dc.keywordspromoting traits
dc.language.isoEnglish
dc.pages111+V
dc.publisherUHF,NAUNI
dc.subBasic Sciences
dc.themeMolecular diversity of rhizobia
dc.these.typePh.D
dc.titleMOLECULAR DIVERSITY OF RHIZOBIA ASSOCIATED WITH LEGUMINOUS TREE SPECIES GROWN UNDER DIFFERENT AGRO-CLIMATIC ZONES OF HIMACHAL PRADESH
dc.typeThesis
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