STUDIES ON THE DEVELOPMENT OF COMMERCIALLY VIABLE FORMULATION OF Agrobacterium radiobacter FOR BIOLOGICAL CONTROL OF CROWN GALL IN STONE FRUIT NURSERIES
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Date
2012
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Abstract
ABSTRACT
The present investigation on “Studies on the development of commercially viable
formulation of Agrobacterium radiobacter for biological control of crown gall in stone fruit
nurseries” was carried out to study the effect of different pH, temperature, incubation period,
broth media and air supply on the growth of native A. radiobacter isolate UHFBA-218 and its
standard strain K-84. The most effective pH was 9 for A. radiobacter strain K-84 and 10 for
A. radiobacter UHFBA-218, whereas the most effective temperature, incubation period, broth
medium and air supply for both the isolates was 25°C , 4 days, yeast extraxt mannitol broth
medium and 20 vv/m, respectively. These parameters were further used for studying the
viability of A. radiobacter isolate UHFBA-218 and strain K-84 in different carrier media –
white stone powder, carboxymethyl cellulose and activated charcoal mixed in 1:2 and 1:5
ratio during six months of storage at room temperature at three different locations viz., main
campus Nauni, Chandigarh and Shimla and its further comparison on the viable counts after
six month of storage at 4°C. White stone powder was superior to other carrier media tested
under these studies. There were appreciable counts ranging from 34.67 to 103.3 × 10 8 cfu/g
after six months of room temperature storage at different locations. However, storage at 4°C
temperature provided maximum viability ranging from 108.50 to 155.7 × 10 8 cfu/g in white
stone powder mixed in 1:5 and 1:2 ratio, respectively.