ISOLATION AND MOLECULAR CHARACTERIZATION OF Pasteurella multocida FROM SWINE

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dc.contributor.advisorSiju Joseph
dc.contributor.authorSREEDEVI. T. K
dc.date.accessioned2020-07-10T06:23:22Z
dc.date.available2020-07-10T06:23:22Z
dc.date.issued2011
dc.description.abstractA total of 193 samples from swine were collected and processed for isolation of P.multocida, from which six isolates could be obtained in pure form. All the isolates were characterized using cultural, morphological and biochemical tests and were identified to be P.multocida. The antibiogram pattern of the isolates revealed that the isolates were resistant to metronidazole, oxacillin and penicillin G. All the isolates were sensitive to chloramphenicol, amoxycillin, ampicillin, cefotaxim, ciprofloxacin, co-trimoxazole, doxycycline, enrofloxacin, erythromycin, gentamicin, nitrofurantoin, norfloxacin and oxytetracycline. The isolates were subjected to PM-PCR and nested PCR for confirming them as P.multocida, which were then tested in a multiplex PCR to identify that all the six isolates belonged to P.multocida serogroup A. These isolates were further characterized genotypically using REP-PCR and REA and observed that REP-PCR and REA using Hpa II generated two profiles which had correlation with phenotypic grouping employing the sugar fermentation tests. Thus in the present study it was found that P.multocida serogroup A was the predominant serovar infecting swine causing pneumonic as well as septicaemic pasteurellosis. It is also concluded that REP-PCR and REA are appropriate tools for conducting molecular analysis of swine P.multocida isolates.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810148968
dc.keywordsISOLATION MOLECULAR CHARACTERIZATION Pasteurella multocida FROM SWINEen_US
dc.language.isoenen_US
dc.pages87en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSURen_US
dc.research.problemISOLATION AND MOLECULAR CHARACTERIZATION OF Pasteurella multocida FROM SWINEen_US
dc.subVeterinary Microbiologyen_US
dc.subjectnullen_US
dc.themeISOLATION AND MOLECULAR CHARACTERIZATION OF Pasteurella multocida FROM SWINEen_US
dc.these.typeM.V.Sc.en_US
dc.titleISOLATION AND MOLECULAR CHARACTERIZATION OF Pasteurella multocida FROM SWINEen_US
dc.typeThesisen_US
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