ISOLATION AND MOLECULAR CHARACTERIZATION OF Pasteurella multocida FROM SWINE
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Date
2011
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR
Abstract
A total of 193 samples from swine were collected and processed for
isolation of P.multocida, from which six isolates could be obtained in pure form.
All the isolates were characterized using cultural, morphological and biochemical
tests and were identified to be P.multocida.
The antibiogram pattern of the isolates revealed that the isolates were
resistant to metronidazole, oxacillin and penicillin G. All the isolates were
sensitive to chloramphenicol, amoxycillin, ampicillin, cefotaxim, ciprofloxacin,
co-trimoxazole, doxycycline, enrofloxacin, erythromycin, gentamicin,
nitrofurantoin, norfloxacin and oxytetracycline.
The isolates were subjected to PM-PCR and nested PCR for confirming
them as P.multocida, which were then tested in a multiplex PCR to identify that
all the six isolates belonged to P.multocida serogroup A.
These isolates were further characterized genotypically using REP-PCR and
REA and observed that REP-PCR and REA using Hpa II generated two profiles
which had correlation with phenotypic grouping employing the sugar
fermentation tests.
Thus in the present study it was found that P.multocida serogroup A was the
predominant serovar infecting swine causing pneumonic as well as septicaemic
pasteurellosis. It is also concluded that REP-PCR and REA are appropriate tools
for conducting molecular analysis of swine P.multocida isolates.
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