MORPHO-METRIC TRAITS AND SSR MARKER ASSAY-BASED DIVERSITY AMONG HOT PEPPER (Capsicum annuum L.) WORKING COLLECTION
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Date
2016-08-17
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Increase use of genetic resources (GR) is a pre-requisite for developing crop
cultivars with broad genetic base which contribute to sustainable production. Precise
information on diversity of GR at phenotypic and genotypic levels help enhance their
enhanced use. Thirty two hot pepper working collection’ genotypes were phenotyped for
morpho-metric traits following field trial at the experimental plot of the department of
Genetics and Plant Breeding (GPB), University of Agricultural Sciences (UAS),
Bengaluru during 2013. They were also assayed using SSR markers at the Hot Pepper
improvement unit, Department of GPB, UAS, Bengaluru. Appreciable magnitudes of
variation was observed for single fruit weight, green fruit yield plant-1, red fruit yield
plant-1, capsaicin and oleoresin contents. The working collection’ genotypes were
grouped into five clusters based on K-means clustering approach. Twenty nine of 50 SSR
markers were polymorphic among the 31 genotypes. The number of alleles per SSR locus
varied from 1 to 4 with an average of 1.8 alleles. Polymorphic Information Content
ranged from 0.00 to 0.64 with an average of 0.21. Four pairs of genotypes; Lampang
Local Short and Wakako Long, Lampang Local Short and CMS 6B, CMS 7B and
Anugraha, Lampang Local Short and LCA 235 were contrasting for productivity per se
traits and polymorphic at greater number of SSR loci. Two pairs of genotypes;
Gouribidanur and LCA 960 and Utkal Awa and LCA 353 were not only polymorphic for
highest number of SSR marker loci but also contrasting for fruit quality traits. These pairs
of genotypes could serve as putative parents in breeding programme to generate
variability and develop segregating populations for SSR marker-assisted mapping
genomic regions controlling productivity per se and fruit quality traits.
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