IN-VITRO STUDIES ON NANOPARTICLE MEDIATED BIODEGRADATION OF ELECTRONIC WASTE BY NATIVE BACTERIA
dc.contributor.advisor | Rai, J. P. N. | |
dc.contributor.author | Chakraborty, Moumita | |
dc.date.accessioned | 2024-03-15T10:46:01Z | |
dc.date.available | 2024-03-15T10:46:01Z | |
dc.date.issued | 2023-02-01 | |
dc.description.abstract | The management of electronic waste (e-waste) becomes a global issue in this digital era. Plastics are neither the main residue nor the most important pollutant in electronic trash, but they take up a lot of room due to their low density and the forms of their parts. They make up about 17 percent of the WEEE (Waste from Electrical and Electronic Equipment) stream. More than 55% of all the polymers in e-waste are made up of ABS (Acrylonitrile Butadiene Styrene) and HIPS (High Impact Polystyrene Sheet). Existing conventional practices are harmful for dealing with e-waste, therefore, indigenous soil bacteria were explored for e-waste treatment through enrichment culture approach followed by screening, identification and their characterization used for in-vitro biodegradation investigation. Soil bacteria were enriched in the presence of e-waste for 6 months and the findings were established through optical density value that were higher in the case of soil enrichment than the control. Based on their morphological, biochemical and molecular characterizations, the bacterial isolates MGP1, MGP4 and MGP15 were identified as Bacillus aryabhattai, Sporosarcina sp., Rhodococcus kroppenstedtii. The selection and biocompatibility testing of potential isolates were performed for the formation of bacterial consortia. All the isolates displayed their best performance at pH 7, temperature 30°C and shaking speed 120 rpm. Supplementing the nutrient medium with additional carbon and nitrogen sources enhanced the rate of polymer degradation by the bacterial isolates. A known bacterial strain Pseudomonas fluorescens was selected for the degradation of the electronic waste and its comparison with that of screened bacterial isolates. Comparative studies showed that e-waste degradation potential of consortium is better than the individual bacterial strains. Titanium Dioxide (TiO2) nanoparticles were used to study its effect on biodegradation ability of the best suited bacterial strain. The biodegradation of e-waste by the selected strains during in vitro experiment was confirmed by analytical processes like FT-IR, FESEM, EDX elemental analysis. Thus, this study besides providing direct and standardized protocol for screening and selection of efficient e-waste utilizing bacteria is also demonstrating potential consortia which are ready to be used. The bacterial isolates were able to degrade e-polymer both in media and soil and therefore can be used profitably for field scale bioremediation technology. | |
dc.identifier.citation | Theses of Ph.D | |
dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810207517 | |
dc.keywords | E-Waste | |
dc.language.iso | English | |
dc.pages | 101p. | |
dc.relation.ispartofseries | 10815 | |
dc.sub | Environmental Science | |
dc.theme | Academic Research | |
dc.these.type | Ph.D | |
dc.title | IN-VITRO STUDIES ON NANOPARTICLE MEDIATED BIODEGRADATION OF ELECTRONIC WASTE BY NATIVE BACTERIA | |
dc.type | Thesis |