STUDIES ON MANAGEMENT OF PULSE BEETLE, Callosobruchus maculatus (Fab.) (COLEOPTERA: CHRYSOMELIDAE) ON STORED CHICKPEA (Cicer arietinum L.) WITH NOVEL TECHNIQUES
| dc.contributor.advisor | T. MADHUMATHI | |
| dc.contributor.author | PANATHULA CHAITHANYA | |
| dc.date.accessioned | 2023-12-02T10:39:24Z | |
| dc.date.available | 2023-12-02T10:39:24Z | |
| dc.date.issued | 2023-12-02 | |
| dc.description.abstract | The present investigation entitled “Studies on management of pulse beetle, Callosobruchus maculatus (Fab.) (Coleoptera: Chrysomelidae) on stored chickpea (Cicer arietinum L.) with novel techniques” was carried out in the laboratory of Department of Entomology, Agricultural College, Bapatla during 2020-21 and 2021-22. A total of twenty chickpea genotypes were screened against C. maculatus through no-choice and free-choice test. The data were recorded on number of eggs laid, adult emergence, mean developmental period, growth index, per cent grain damage by count and weight, per cent weight loss, number of exit holes on grains. The chickpea genotypes were categorized based on mean and standard deviation of different parameters studied through free-choice test as less susceptible (NBeG 452, NBeG 1129, ICC 86111, NBeG 49 and NBeG 776), moderately susceptible (NBeG 3, NBeG 47, JG 11, NBeG 699, NBeG 779, NBeG 857, NBeG 1137, NBeG 1174, NBeG 119, NBeG 810, KAK 2 and Vihar) and highly susceptible (NBeG 440, NBeG 789 and NBeG 833). Certain physical parameters and biochemical constituents of chickpea genotypes also influenced their relative susceptibility to C. maculatus. The less susceptible genotypes were desi type, small to medium sized grains with wrinkled surface nature. NBeG 452 and NBeG 1129 were found with high phenol content (119.06 and 115.71 mg CAE 100 g-1), tannin content (5.46 and 4.94 mg TAE g-1) and low total soluble sugars (55.94 and 59.98%), protein content (20.16 and 18.67%), medium test weight (24.73 and 25.27 g) and high seed coat thickness (0.173 and 0.207 mm); ICC 86111 with less test weight (17.27 g) recorded low number of eggs (24.33, 25.33 and 25.67) and adult emergence (19.67, 19.00 and 20.67) per 100 grains, low growth index (2.89, 2.73 and 2.90), lesser grain damage (17.75, 18.18 and 19.89%), low weight loss (4.86, 4.35 and 3.78%) and minimum number of exit holes per ten grains (2.67, 2.67 and 3.00) and less susceptible genotype, NBeG 49 was xviii found with high phenol content (118.91 mg CAE 100 g-1) and tannin content (5.15 mg TAE g-1), NBeG 776 with more seed coat thickness (0.210 mm) and less total soluble sugars (60.11%). Whereas highly susceptible genotypes were kabuli type, medium to bold sized grains with smooth surface nature, apart from these, NBeG 440 and NBeG 789 were found with less phenol content (84.48 and 74.25 mg CAE 100 g-1), tannin content (2.75 and 2.23 mg TAE g-1), more total soluble sugars (75.36 and 73.65%), medium test weight (31.33 and 35.67 g), less seed coat thickness (0.060 and 0.103 mm) and NBeG 833 with more test weight (44.60 g), low seed coat thickness (0.093 mm), more total soluble sugars (72.53%) and low tannin content (3.06 mg TAE g-1). Bioassay studies through jute cloth disc impregnation method revealed the lowest LC50 (0.00006%), LC75 (0.0002%) and LC90 (0.0006% and 0.0008%) values for emamectin benzoate and chlorfenapyr at 72 HAT and found relatively more toxic as compared to malathion (233.33, 191.00; 156.50 and 117.38 times, respectively) and deltamethrin (41.67, 83.00; 151.50 and 113.63 times, respectively). Permethrin @ 0.14% was found to be most effective against C. maculatus with highest average toxicity (62.38%) and with high PT index (11228.57) and recording relatively minimum population build-up of C. maculatus and per cent grain damage and weight loss of chickpea grains up to 150 DAT. However, among the novel insecticides, spinosad @ 0.1% was found to be effective with an average toxicity of 39.05 per cent against C. maculatus with 5857.14 PT index. The microwave radiation treatment of pre infested chickpea grains at 900-Watt power level with 30 s exposure time was found to be effective with less number of adult emergence of C. maculatus per 100 grains (6.67) with prolonged mean development period (30.01 days), low per cent adult emergence (17.32%), growth index (0.58) and number of exit holes per ten g grains (6.00). The microwave treatment, 720 W 30 s was found to be optimum in controlling growth and development of pulse beetle with 15.67 number of adults emerged per 100 g sample and 0.64 growth index along with maintenance of viability and germination above 80 per cent, followed by treatment at 540 W 30 s found to be effective in maintaining the viability and germination percentages above 90 per cent with 22.33 number of adults per 100 g sample and 0.69 growth index. Among the immature stages of C. maculatus, pupa was found to be resistant to microwave radiation with respect to highest range of per cent adult emergence of C. maculatus from the irradiated chickpea samples containing pupal stages (0.00 to 79.33%) followed by late larval (0.00 to 74.67%), early larval (0.00 to 72.00%), and egg stage (0.00 to 69.33%) at 900 W 90 s to 180 W 30 s, respectively. Chickpea grains stored in super bags recorded zero number of adult beetles of C. maculatus, zero grain damage by count and weight, zero weight loss, no moisture change, and above 90 per cent viability and germination of chickpea grains up to six months storage period followed by triple layer plastic bags and found to be most effective when compared to other types of bags tested. | |
| dc.identifier.other | D6410 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810202027 | |
| dc.keywords | PULSE BEETLE | |
| dc.keywords | Callosobruchus maculatus | |
| dc.keywords | STORED CHICKPEA | |
| dc.language.iso | English | |
| dc.pages | 258 | |
| dc.publisher | Acharya N G Ranga Agricultural University | |
| dc.relation.ispartofseries | D6410; D6410 | |
| dc.sub | Entomology | |
| dc.theme | STUDIES ON MANAGEMENT OF PULSE BEETLE, Callosobruchus maculatus (Fab.) (COLEOPTERA: CHRYSOMELIDAE) ON STORED CHICKPEA (Cicer arietinum L.) WITH NOVEL TECHNIQUES | |
| dc.these.type | Ph.D | |
| dc.title | STUDIES ON MANAGEMENT OF PULSE BEETLE, Callosobruchus maculatus (Fab.) (COLEOPTERA: CHRYSOMELIDAE) ON STORED CHICKPEA (Cicer arietinum L.) WITH NOVEL TECHNIQUES | |
| dc.type | Thesis |