EVALUATION OF TRANSGENIC GROUNDNUT PROGENIES FOR TIKKA DISEASE RESISTANCE
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Date
2007-09-19
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK ,BANGALORE
Abstract
Groundnut transgenics {Arachis hypogaea L.) cv. TMV-2 expressing
glucanase, NPRl/Defensin and combination of both the genes were developed
using an Agrobacterium tumefaciens (LBA 4404/pKVD 4 for glucanase and GV
2260/pCAMBIA 2300 for NPRl/Defensin) mediated in planta transformation, a
tissue culture-independent method. PGR analysis of the genomic DNA isolated
from T2 and T3 generation transgenics showed the integration of transgenes in the
genome of the groundnut plants. The expression of the heterologous glucanase,
NPRl/Defensin and combination of both the genes (NPRl/Defensin in glucanase
transformants) driven by CaMV 35S promoter led to a high level of activity in
some of the transgenic plants. Pot culture experiments indicated increased ability
of these plants to resist tikka disease. These results suggest that a heterologous
glucanase, NPRl/Defensin and combination of both the genes (NPRl/Defensin +
glucanase) were functional in groundnut and expressed in healthy plants. The
genomic Southern analysis showed the integration and stability of transgenes in
the groundnut plants. The segregation pattern revealed that the transgenes
followed the Mendalian pattern of inheritance of monohybrid ratio 3:1. Based on
in-vitro and in-vivo bioassay against Cercospora spp. the resistant plants were
selected. The transgenics expressing glucanase were three plants, where
transgenics expressing NPRl/Defensin and NPRl/Defensin+glucanase were four
and nine plants respectively
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