Molecular characterization of cluster bean (Cyamopsis tetragonoloba L. Taub.) genotypes
Loading...
Date
2019
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CCSHAU
Abstract
Cluster bean (Cyamopsis tetragonoloba L. Taub.) is an annual, diploid legume crop with 14 number of chromosomes and highly valued for presence of galactomannan in its seeds. For commercial use of seeds, their genetic purity determination is very important. The present study was carried out for development of molecular profile of commercial varieties of cluster bean using SSRs and to study molecular diversity in selected genotypes of cluster bean using EST-SSRs. For this study, seeds of 22 commercial varieties and 41 germplasm lines of guar were grown in net house of the department and Farm area of Forage Section, Department of Genetics and Plant Breeding. DNA was extracted using CTAB method. Molecular analysis was carried out using 79 SSRs which included 40 EST-SSRs and 39 miSSRs from Medicago truncatula and cluster bean (developed in our lab). Out of 79 SSRs, 55 SSRs were found polymorphic and employed for varietal identification. Among these polymorphic SSRs, eight SSRs identified selected varieties. Ct-miSSR-17, Ct-miSSR-19, Mt-miRNA-SSR45, Mt-miRNA-SSR47 and Mt-miRNA-SSR164, EST-SSR2, EST-SSR17 and EST-SSR40 identified selected varieties. Among 55 polymorphic SSRs, size of amplified products ranged from 100-1160 bp and PIC value ranged from 0.28 to 0.91. The molecular analysis divided the 22 cluster bean varieties into two major clusters at similarity coefficient of 0.06 and HG 6 existed as an isolated genotype. Cross-transferability of Medicago truncatula miRNA-SSRs showed 31% cross-transferability among cluster bean varieties. Molecular analysis showed that IC 402295-2, IC 522486, IC 113452, IC 415161 and IC 113489 were extreme and diverse group with around 30 % gum content while IC 113472 and IC 370725 were low gum (<20%) genotypes. The molecular analysis of 63 genotypes together showed that all the 22 varieties are in different genetic pool than 41 germplasm lines and can be exploited in cluster bean breeding programme.
Description
Keywords
null