DESIGNING AND VALIDATION OF PRIMERS FOR HIGH GRAIN NUMBER FOR USE IN MOLECULAR BREEDING IN RICE

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dc.contributor.advisorBehera, L
dc.contributor.authorSwain, Pranati
dc.date.accessioned2017-01-16T10:45:39Z
dc.date.available2017-01-16T10:45:39Z
dc.date.issued2014
dc.description.abstractImprovement of rice grain yield is an important goal in rice breeding. Yield improvement efficiency depends upon yield related components like grain number per panicle, grain weight, tillers number per plant, etc. Of these factors, grain number per panicle was shown to be highly correlated with yield. Pyramiding genes for grain number is highly indispensable in rice improvement programs. The high grain number genes, Apo1, Dep1, Ghd7, Gn1a, fzp, lax1, rcn2 and Sp1 are responsible for more seed production leading to increase in grain yield in rice. The length, sequence ID, chromosomal position and protein evidences of these genes were found from Orzabase, RAP-DB and Gramene database. The promoter, poly-A signal, DNA strand, reading frame, initial signal/3’ splice site score, 5’ splice site, coding region, probability of exon and intron of high grain number genes under study were found out. These informations were used to design primers for these high grain number genes. The study of secondary structure of proteins indicated that Gn1a, rcn2 and Sp1 were found to be better and can be used for further study like protein- protein interactions involved in expression of high grain number in rice. The Ramachandran plot study indicated that the Gn1A protein is reviewed while the proteins of rcn2 and Sp1 are un-reviewed. The similarity search of designed primers showed 100% similarity with respective high grain number genes. Three deigned primers for Dep1gene, one for Ghd1 gene and one for Apo1 gene were tested for amplification from high and low grain rice varieties, HGN1 and Heera, respectively. Two primers, Dep1-1 (promotor) for Dep1gene and Ghd7-2(Exon1) for Ghd7 gene amplified fragments from high grain variety, HGN1 while failed to amplify from low grain variety, Heera, indicating that these primers would be useful for introgression of genes from high grain varieties into low grain varieties to improve yield potential in rice through molecular breeding approach in future.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/96426
dc.language.isoenen_US
dc.relation.ispartofseriesTh;4256
dc.subBioinformaticsen_US
dc.these.typeM.Sc
dc.titleDESIGNING AND VALIDATION OF PRIMERS FOR HIGH GRAIN NUMBER FOR USE IN MOLECULAR BREEDING IN RICEen_US
dc.typeThesisen_US
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