HISTOMORPHOLOGICAL CHANGES IN THE TESTIS OF RATS EXPOSED TO METHYL MERCURY IN RELATION TO PITUITARY-TESTICULAR AXIS
| dc.contributor.advisor | NAGAMALLESWARI, Y(MAJOR) | |
| dc.contributor.author | KARUNA SRI, VADDI | |
| dc.contributor.other | KISHORE, P V S | |
| dc.date.accessioned | 2017-05-20T10:34:32Z | |
| dc.date.available | 2017-05-20T10:34:32Z | |
| dc.date.issued | 2014-10 | |
| dc.description | THESES | en_US |
| dc.description.abstract | ABSTRACT: This study was conducted on 36 adult male Sprague Dawley rats of 6 weeks age. These subjects were randomly assigned into three groups, viz. control (group I), 2.5ppm methyl mercury exposure (group II) and 5.0ppm methyl mercury exposure (group III). Methyl mercury was fed in the form of methyl mercuric chloride in drinking water daily. There was a significant increase in body weight in the 5.0ppm group than in the 2.5ppm and control groups at 35 days of exposure. Elongated bean shaped testes were present. The average total testes weight also increased significantly in both the treated groups than in the control group at 35 days post exposure. The average length and width of both right and left testes increased in 2.5 and 5.0ppm groups when compared to the control at 14 days of exposure. In the high dose (5ppm) group there was a significant increase in the average width of the right and left testis compared to the control at 35 days post exposure. The length and width of the left testis in both exposure groups was significantly increased.There was a significant increase in the mean sperm count in rats of 2.5ppm group than in other groups at 14 and 35 days post exposure. The average number of head and tail abnormalities also increased in both treated groups during the same period. The average serum total testosterone level increased significantly in the 5.0ppm group than in 2.5ppm and in the control groups at 14 days post exposure. However, there was no significant increase in the serum total testosterone level among all the three groups at 35 days post exposure.The thickness of tunica albuginea (capsule) of testis was reduced in both the treated groups (2.5 and 5.0ppm) at 14 and 35 days post exposure. A potential space was seen between the basal and adluminal compartments at 14 days post exposure in both the treatment groups. No potential space was observed in the control group. Hypertrophy and vacuolation of sertoli cells were present in both the treated groups at 14 and 35 days post exposure. Normal cohort of 5-6 types of cell arrangement was lost in both the treated groups at 14 and 35 days post exposure. Mitotic divisions were seen in the group given 5ppm at 14 days post exposure and in both the treated groups at 35 days post exposure. Thinning and degeneration of the peritubular membrane was found in both treatments at both the exposure periods. Altogether, the above changes lead to disruption of blood-testis barrier. Hypertrophy and vacuolation of leydig cells were observed in the interstitium of testis in both the treatment groups compared to the control at 35 days post exposure.The average weight, length and width of pituitary glands increased in both treated groups compared to the control and it was statistically significant at 14 days post exposure. Hypertrophy of B1 (LH cells) and B2 (FSH cells) was observed in the treated groups of both exposures compared to the control. B2 cells were present in clusters at the postero-lateral aspect of pars distalis. Some cells showed a centrally placed nucleus while others had a peripheral nucleus. Vacuoles were present on the apical surface of nucleus which might represent the Golgi complex in the 2.5ppm group. This might be an indication of the secretory state of cells. No vacuoles were found in the control group. Hypertrophy of sub capsular capillaries was observed at the periphery of pars distalis. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810011840 | |
| dc.keywords | RATS; TESTIS; METHYL MERCURY; PITUITARY-TESTICULAR AXIS | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 109 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI – 517 502. (A.P) INDIA | en_US |
| dc.sub | Veterinary Anatomy | en_US |
| dc.subject | null | en_US |
| dc.theme | HISTOMORPHOLOGICAL CHANGES IN THE TESTIS OF RATS EXPOSED TO METHYL MERCURY IN RELATION TO PITUITARY-TESTICULAR AXIS | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | HISTOMORPHOLOGICAL CHANGES IN THE TESTIS OF RATS EXPOSED TO METHYL MERCURY IN RELATION TO PITUITARY-TESTICULAR AXIS | en_US |
| dc.type | Thesis | en_US |